Glutamate and N-methyl-D-aspartate binding sites in the guinea pig hippocampus: ontogeny and effect of acute in vitro ethanol exposure

Alcohol
S Abdollah, J F Brien

Abstract

The objectives of this study were to characterize the ontogeny of the L-glutamate (glutamate) and N-methyl-D-aspartate (NMDA) binding sites in the developing guinea pig hippocampus, and to determine the effect of acute in vitro ethanol exposure on these binding sites. Specific [3H]glutamate binding and NMDA-sensitive [3H]glutamate binding were determined using a guinea pig hippocampal synaptic membrane preparation (HSMP). To characterize the ontogeny of the density (Bmax) and affinity (Kd) of the glutamate and NMDA binding sites, saturation analysis was conducted on HSMP of guinea pigs at gestational day (GD) 50 (immature fetus; term, GD 68), GD 62 (mature, near-term fetus), postnatal day (PD) 13 (neonate), and PD > 60 (adult). To examine the effect of ethanol on the glutamate and NMDA binding sites, HSMP of guinea pigs at GD 50, GD 62, PD 13, and PD > 60 was incubated with ethanol (0-100 mM), followed by determination of specific [3H]glutamate binding and NMDA-sensitive [3H]glutamate binding. To determine the effect of 50 mM ethanol on the Bmax and Kd of the glutamate and NMDA binding sites, HSMP of guinea pigs at GD 62 and PD > 60 was incubated with 0 or 50 mM ethanol followed by saturation analysis. The Bmax values of the hi...Continue Reading

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Citations

Oct 30, 2004·Hippocampus·Sandra GuidiRenata Bartesaghi
Apr 29, 1998·Brain Research. Developmental Brain Research·K A Kimura, J F Brien
Mar 5, 1999·Brain Research. Developmental Brain Research·C D BaileyJ N Reynolds
Jul 16, 1999·Neurochemistry International·H J Walter, R O Messing
May 22, 2003·Neurotoxicology and Teratology·Michelle L ByrnesJames F Brien
Apr 30, 2004·Biology of Reproduction·Dawn OwenStephen G Matthews
Jun 1, 2000·Alcoholism, Clinical and Experimental Research·E T CostaC F Valenzuela
Sep 16, 2000·The Journal of Biological Chemistry·E T CostaC F Valenzuela

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