Glutamate-dependent transcriptional regulation of GLAST: role of PKC

Journal of Neurochemistry
Esther López-Bayghen, Arturo Ortega

Abstract

The Na+-dependent glutamate/aspartate transporter GLAST plays a major role in the removal of glutamate from the synaptic cleft. Short-term, as well as long-term changes in transporter activity are triggered by glutamate. An important locus of regulation is the density of transporter molecules present at the plasma membrane. A substrate-dependent change in the translocation rate of the transporter molecules accounts for the short-term effect, whereas the long-term modulation apparently involves transcriptional regulation. Using cultured chick cerebellar Bergmann glial cells, we report here that glutamate receptors activation mediate a substantial reduction in the transcriptional activity of the chglast promoter through the Ca2+/diacylglicerol-dependent protein kinase (PKC) signaling cascade. Overexpression of constitutive active PKC isoforms of mimic the glutamate effect. Accordingly, increased levels of c-Jun or c-Fos, but not Jun-B, Jun-D or Fos-B, lower the chglast promoter activity. Serial deletions and electrophorectic mobility shift assays were used to define a specific region within the 5' proximal region of the chglast promoter, associated with transcriptional repression. A putative glutamate response element could be de...Continue Reading

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Citations

Jul 5, 2006·The Cerebellum·Tomas C Bellamy
May 24, 2011·Toxicological Sciences : an Official Journal of the Society of Toxicology·Yaneth Castro-CoronelEsther López-Bayghen
Jan 23, 2009·American Journal of Physiology. Endocrinology and Metabolism·Harinder S Hundal, Peter M Taylor
Sep 8, 2010·Neurochemistry International·Iliana BarreraArturo Ortega
Jan 13, 2009·Neuroscience Letters·Irais Poblete-NaredoArturo Ortega
Mar 14, 2008·Neurochemistry International·Gisela AguirreArturo Ortega
Jun 22, 2006·Journal of Neurochemistry·Esther López-BayghenArturo Ortega
Mar 31, 2007·Journal of Neurochemistry·Sandra RosasArturo Ortega
Sep 9, 2011·IUBMB Life·Esther López-Bayghen, Arturo Ortega
Sep 20, 2015·Neurochemical Research·Alain M GuillemArturo Ortega
May 18, 2016·Neurochemistry International·Orquidia G Mendez-FloresArturo Ortega
Nov 23, 2017·Journal of Neurochemistry·Ivan DomithRoberto Paes-de-Carvalho
May 18, 2005·Neurochemical Research·Roque Galaz-VegaArturo Ortega
Jun 2, 2005·Journal of Neuroscience Research·J Alfredo MéndezArturo Ortega
Jul 12, 2019·Neurotoxicity Research·Ada G Rodríguez-CampuzanoArturo Ortega
May 5, 2020·Journal of Biochemical and Molecular Toxicology·Catya Jiménez-TorresArturo Ortega
Aug 27, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Thomas KornStefan Jung
May 16, 2021·Neuropharmacology·Ada G Rodríguez-Campuzano, Arturo Ortega

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