Glutamate-dependent translational control through ribosomal protein S6 phosphorylation in cultured bergmann glial cells

Neurochemical Research
Marco Flores-MéndezArturo Ortega

Abstract

Glutamate (Glu) the main excitatory neurotransmitter of the central nervous system regulates gene expression at different levels through the activation of specific membrane receptors and transporters expressed in neurons and glia cells. A membrane to nucleus signaling cascade triggered by this neurotransmitter has been described in cultured cerebellar Bergmann glia cells isolated from chick embryos. Furthermore, it has also been described that Glu receptors activation is linked to a modulation of [(35)S]-methionine incorporation into newly synthesized polypeptides. In order to gain insight into the signal transduction cascades that participate in this effect, in the present study we characterized the phosphorylation of a critical component of the translational machinery, namely the ribosomal protein S6. The phosphorylation sites in rpS6 have been mapped to five clustered residues, Ser235, Ser236, Ser240, Ser244 and Ser247. Nevertheless, Ser236 phosphorylation is the primary phosphorylation site. The kinases responsible of this modification are p70(S6K) and p90(RSK). rpS6 phosphorylation increases the affinity of 40s subunit for mRNAs and thus facilitates translational initiation. Glutamate exposure of cultured cerebellar Bergma...Continue Reading

References

Jan 1, 1991·Neuroscience·A OrtegaV I Teichberg
Jan 1, 1994·Annual Review of Neuroscience·M Hollmann, S Heinemann
Oct 6, 1998·Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie·C Pittenger, E Kandel
Jun 29, 2000·Trends in Pharmacological Sciences·V Gallo, C A Ghiani
Sep 19, 2002·Journal of Neuroscience Research·Adán AguirreArturo Ortega
Dec 7, 2002·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Victoria Coutinho, Thomas Knöpfel
May 14, 2003·Trends in Cell Biology·Herbert Tschochner, Ed Hurt
Sep 17, 2004·Journal of Neuroscience Research·Alejandro MillánArturo Ortega
Nov 20, 2004·Nature Reviews. Neuroscience·Eric Klann, Thomas E Dever
Apr 14, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Daoying HuEric Klann
May 9, 2006·Trends in Biochemical Sciences·Igor Ruvinsky, Oded Meyuhas
Jun 22, 2006·Journal of Neurochemistry·Lasse K BakHelle S Waagepetersen
Jan 29, 2008·Neurochemistry International·Iliana BarreraArturo Ortega
Jul 19, 2008·Neuron Glia Biology·Esther López-BayghenArturo Ortega
Aug 16, 2008·International Review of Cell and Molecular Biology·Oded Meyuhas
Jul 7, 2009·Neurochemistry International·Rossana C ZepedaArturo Ortega
Nov 10, 2009·Brain Research Reviews·Gertrudis Perea, Alfonso Araque
Nov 18, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Robert J SalmondRose Zamoyska
Sep 8, 2010·Neurochemistry International·Iliana BarreraArturo Ortega
Sep 3, 2011·Cell·Marta Navarrete, Alfonso Araque
Oct 27, 2011·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Luc Pellerin, Pierre J Magistretti
Dec 5, 2012·Cold Spring Harbor Perspectives in Biology·John W B HersheyMichael B Mathews
Mar 29, 2013·Neurochemical Research·Marco A Flores-MéndezArturo Ortega
Jan 15, 2014·The Journal of Physiology·Saju BalakrishnanTomas C Bellamy

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.