PMID: 9530923Apr 8, 1998Paper

Glutamate induces hydroxyl radical formation in vivo via activation of nitric oxide synthase in Sprague-Dawley rats

Neuroscience Letters
E LancelotJ Callebert

Abstract

It was recently reported that neuronal nitric oxide synthase (NOS) generates oxygen-derived free radicals in vitro at low concentrations of L-arginine. Using the microdialysis technique, we monitored both hydroxyl radical (.OH) and nitric oxide (.NO) formation in rat striatum perfused with glutamate (500 mM). .OH and .NO were quantitated in microdialysates by measuring the amounts of the non-enzymatic hydroxylation product of salicylate (2,3-dihydroxybenzoic acid) and the metabolites of .NO (nitrite + nitrate), respectively. .OH levels were dramatically increased during glutamate perfusion, while .NO generation was virtually abolished. .OH production was inhibited by the specific NOS blocker, NG-nitro-L-arginine methyl ester. This effect was not reversed but potentiated by L-arginine. Thus, it is likely that NOS generates oxygen-derived free radicals instead of .NO in brain subjected to highly excitotoxic conditions.

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Citations

Nov 27, 1999·Life Sciences·C M LauderbackD A Butterfield
Sep 6, 2000·Pharmacology, Biochemistry, and Behavior·G M CunhaG S Viana
Nov 27, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Fu-Chou ChengTung-Hu Tsai
May 8, 2003·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Yoshitaka HirookaAkira Takeshita
Feb 9, 2005·Neuroscience Letters·Luiz Fernando Freire RoyesCarlos Fernando Mello
Oct 18, 2005·Clinical Neurology and Neurosurgery·Amit AgrawalMurali Manju
Jul 19, 2011·Neurotoxicology and Teratology·R Lugo-HuitrónV Pérez-De La Cruz
Oct 3, 2003·Journal of Neurophysiology·Jason J KuoAlfons B A Kroese
May 28, 2020·The European Journal of Neuroscience·César Augusto BrüningCristina Wayne Nogueira

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