Glutamate quantification by PRESS or MEGA-PRESS: Validation, repeatability, and concordance

Magnetic Resonance Imaging
Tamar M van VeenendaalJacobus F A Jansen

Abstract

While PRESS is often employed to measure glutamate concentrations, MEGA-PRESS enables simultaneous Glx (glutamate and glutamine) and GABA measurements. This study aimed to compare validation, repeatability, and concordance of different approaches for glutamate quantification at 3T to aid future studies in their selection of the appropriate sequence and quantification method. Nine phantoms with different glutamate and glutamine concentrations and five healthy participants were scanned twice to assess respectively the validation and repeatability of measurements with PRESS and MEGA-PRESS. To assess concordance between the different methods, results from 95 human participants were compared. PRESS, MEGA-PRESS (i.e. difference), and the MEGA-PRESS OFF spectra were analyzed with both LCModel and Gannet. In vitro, excellent agreement was shown between actual and measured glutamate concentrations for all measurements (r>0.98). In vivo CVs were better for PRESS (2.9%) than MEGA-PRESS (4.9%) and MEGA-PRESS OFF (4.2%). However, the concordance between the sequences was low (PRESS and MEGA-PRESS OFF, r=0.3) to modest (MEGA-PRESS versus MEGA-PRESS OFF, r=0.8). Both PRESS and MEGA-PRESS can be employed to measure in vivo glutamate concentrat...Continue Reading

Citations

Oct 12, 2018·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Christian Stefan LegindBirte Yding Glenthøj
Jan 22, 2019·NMR in Biomedicine·Elvisha DhamalaJamie Near
Jul 11, 2019·Journal of Neurophysiology·Reuben RideauxAndrew E Welchman
Jan 29, 2020·NMR in Biomedicine·Tiffany BellAshley D Harris
Dec 5, 2019·NeuroImage. Clinical·John-Paul NicoloPatrick Kwan
Sep 4, 2021·Psychiatry Research. Neuroimaging·Kate E M GodfreyNicholas Hoeh

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