Glutamate receptor and transporter modifications in rat organotypic hippocampal slice cultures exposed to oxygen-glucose deprivation: the contribution of cyclooxygenase-2

Neuroscience
I L LlorenteA Fernández-López

Abstract

Meloxicam is a non-steroidal anti-inflammatory drug which has been reported to lessen the ischemic transcriptional effects in some of the glutamatergic system genes as well as to decrease the infarct volume in in vivo assays. In this study, we show how the presence of meloxicam decreases cell mortality in assays of oxygen-glucose deprivation (OGD) in rat organotypic hippocampal slices culture. Mortality was measured using propidium iodide. Transcript levels of some glutamatergic system genes, including vesicular and membrane glutamate transporters (VGLUT1, VGLUT2, GLAST-1A, GLT-1, and EAAC-1) and some glutamatergic receptor subunits (NMDA receptor, GluN1, GluN2A and GluN2B subunits and AMPA receptor, GluA1 and GluA2 subunits) were measured by real-time PCR (qPCR). The transcription of vesicular glutamate transporters and glutamatergic receptor subunits, but not membrane glutamate transporters, was modified by the presence of meloxicam. The study demonstrates the neuroprotective role of meloxicam in organotypic hippocampal slice cultures and shows how meloxicam is able to selectively increase or decrease the OGD-induced changes in the expression of the different glutamatergic system genes studied here. We suggest that the neurop...Continue Reading

References

Dec 1, 1990·Italian Journal of Neurological Sciences·A R MassaroP Tonali
Jan 1, 1994·Annual Review of Neuroscience·M Hollmann, S Heinemann
Nov 5, 1997·Trends in Neurosciences·D E Pellegrini-GiampietroR S Zukin
May 23, 1998·Annual Review of Pharmacology and Toxicology·J R VaneR M Botting
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·M NakayamaS H Graham
May 10, 2001·Current Opinion in Cell Biology·P Mitchell, D Tollervey
Feb 8, 2002·Molecular Neurobiology·R Sattler, M Tymianski
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jan 13, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Louise McCulloughKatrin Andreasson
Nov 25, 2006·Disease Markers·Gavin Giovannoni
Jan 16, 2007·Brain Research Reviews·Suresh L MehtaRam Raghubir
Jan 31, 2007·Stroke; a Journal of Cerebral Circulation·Aaron M HambyRaymond A Swanson
Nov 23, 2007·Neuropharmacology·Sam M Greenwood, Christopher N Connolly
Oct 29, 2008·The Veterinary Clinics of North America. Small Animal Practice·Mark G Papich
Oct 1, 1998·Journal of Clinical Rheumatology : Practical Reports on Rheumatic & Musculoskeletal Diseases·S J Vane
Jul 15, 2009·British Journal of Pharmacology·Laetitia MonyPierre Paoletti
Mar 11, 2010·Methods : a Companion to Methods in Enzymology·Sean TaylorMarie Nguyen
Mar 31, 2010·Neurochemistry International·Sheyla MontoriArsenio Fernández-López
Apr 23, 2010·Neuropathology and Applied Neurobiology·S MontoriB Martínez-Villayandre
Aug 19, 2010·Pharmacological Reviews·Stephen F TraynelisRay Dingledine
Sep 16, 2010·Nature Reviews. Neuroscience·Giles E Hardingham, Hilmar Bading
Mar 15, 2011·The European Journal of Neuroscience·Pierre Paoletti
May 24, 2011·Molecular and Cellular Neurosciences·Clare M Gladding, Lynn A Raymond
Jul 13, 2011·Frontiers in Molecular Neuroscience·Myriam GorospeMircea Ivan
Aug 3, 2011·Neurobiology of Disease·Andrea C LoboCarlos B Duarte
Jan 17, 2012·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Juan Antonio López-VillodresJosé Antonio González-Correa
Feb 22, 2012·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Antonio Sanz-ClementeKatherine W Roche
May 21, 2013·Nature Reviews. Neuroscience·Pierre PaolettiQiang Zhou

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Citations

Nov 16, 2019·International Journal of Molecular Sciences·Anna R Malik, Thomas E Willnow
Oct 11, 2019·Neuropharmacology·Damiana CavalloDomenico E Pellegrini-Giampietro

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