PMID: 9419013Jan 7, 1998Paper

Glutamate receptor-mediated calcium responses in acutely isolated hippocampal astrocytes

Glia
Z Cai, H K Kimelberg

Abstract

GFAP(+) cells were acutely isolated from the hippocampi of 1-10 day old rats, and the intracellular calcium responses to L-glutamate, ATP, and 5-HT were studied in single cells. Eighty-two percent of such cells responded to glutamate, 20% of them responded to ATP, and none responded to 5-HT. The same cells that failed to respond to ATP and 5-HT often responded to glutamate. These proportions of cells responding to glutamate and ATP are very similar to those reported for GFAP(+) astrocytes in hippocampal slices (Porter and McCarthy, 1995a,b). After culturing for 1-2 days in serum-containing medium, 60% of such acutely isolated cells responded to either glutamate or ATP, and 5% to 5-HT. After 1 week in culture, the percentage of cells responding to glutamate remained essentially the same (62%) but the percentages of cells responding to ATP and 5-HT increased to 92% and 62%, respectively. These percentages were very close to the results obtained from primary hippocampal astrocyte cultures prepared from 1 day old rats and cultured for 1-2 weeks in vitro. Pharmacological characterization showed that the Ca2+ responses of acutely isolated hippocampal astrocytes from P1-10 rats was due to activation of a group I metabotropic glutamate...Continue Reading

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Citations

Mar 25, 2000·Neurochemistry International·H K KimelbergM Zhou
Jun 19, 2002·Biochimica Et Biophysica Acta·Marion R SteinerSheldon M Steiner
Jul 11, 2002·American Journal of Physiology. Cell Physiology·Alexander A Mongin, Harold K Kimelberg
Jan 20, 2018·Physiological Reviews·Alexei Verkhratsky, Maiken Nedergaard
Feb 16, 2019·Neurochemical Research·Alexei Verkhratsky, Alexandr Chvátal
Jan 17, 2019·International Journal of Molecular Sciences·Katarzyna SkowrońskaJan Albrecht

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