Glutamate transporter localization does not correspond to the temporary functional recovery and late degeneration after acute ocular ischemia in rats

Experimental Eye Research
N L Barnett, Sinisa D Grozdanic

Abstract

To determine whether the localization of retinal glutamate transporters is affected by retinal ischaemia and whether their ability to transport glutamate decreases with the progression of ischemic retinal and optic nerve degeneration. Retinal ischemia was induced in rats by acutely increasing the intraocular pressure (IOP, 110 mmHg/60 min). Reperfusion was permitted for periods up to 60 days post-ischemia. Functional evaluation was performed by monitoring the pupil light reflexes (PLRs) and electroretinograms (flash, flicker ERG and oscillatory potentials). Glutamate transporter localization and D-aspartate (glutamate analogue) uptake were assessed by immunohistochemistry. Intense immunoreactivity for the retinal glutamate transporters (GLAST, GLT1, EAAC1 and EAAT5) was observed at all time points after the insult, despite severe retinal degeneration. D-aspartate was also normally accumulated in the ischemic retinas. Ten days post-operatively the PLR ratio (ratio=indirect/direct PLR=34+/-7.5%) was significantly less than the pre-operative value (pre-op=76.7+/-2.6%, p<0.05). However, 25 and 35 days post-operatively PLR ratios did not differ significantly from pre-operative values (44.4+/-6.9 and 53.8+/-9.6%, p>0.05). Forty-five ...Continue Reading

References

May 1, 1995·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·R A SwansonR P Simon
Apr 29, 1998·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·A AkaikeK Kaneda
Sep 21, 2000·Experimental Eye Research·J M Kwong, T T Lam
Jun 18, 2002·Journal of Neurochemistry·Natalie D Bull, Nigel L Barnett
Feb 14, 2003·Archives of Ophthalmology·Robert A HonkanenYoung H Kwon
May 27, 2003·Investigative Ophthalmology & Visual Science·Sinisa D GrozdanicIoana M Sonea

❮ Previous
Next ❯

Citations

Mar 17, 2010·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Sinisa D GrozdanicDonald S Sakaguchi
Nov 19, 2009·Visual Neuroscience·Leith Moxon-LesterNigel L Barnett
May 4, 2010·Experimental Eye Research·David J HolcombeNigel L Barnett
Dec 31, 2008·Neurochemistry International·Andreas BringmannAndreas Reichenbach
Mar 14, 2008·Experimental Eye Research·Hideaki OkumichiTakashi Kanamoto
Aug 31, 2007·The Journal of Comparative Neurology·Daniel SunMichael Kalloniatis
Aug 31, 2007·The Journal of Comparative Neurology·Daniel SunMichael Kalloniatis
Dec 24, 2010·Clinical & Experimental Ophthalmology·David J GunnNigel L Barnett
Jan 12, 2017·Expert Opinion on Drug Discovery·Renu Agarwal, Puneet Agarwal
Aug 19, 2007·Journal of Glaucoma·Iok-Hou Pang, Abbot F Clark

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis