PMID: 7546749Sep 1, 1995Paper

Glutamate transporters in glial plasma membranes: highly differentiated localizations revealed by quantitative ultrastructural immunocytochemistry

Neuron
F A ChaudhryJ Storm-Mathisen

Abstract

The glutamate transporters GLT-1 and GLAST were studied by immunogold labeling on ultrathin sections of rat brain tissue embedded in acrylic resins at low temperature after freeze substitution. Both proteins were selective markers of astrocytic plasma membranes. GLT-1 was much higher in hippocampal astrocytes than in cerebellar astrocytes. Astroglial membrane GLAST densities ranked as follows: Bergmann > cerebellar granular layer approximately hippocampus > cerebellar white matter. No astrocyte appeared unlabeled. Astrocytic membranes facing capillaries, pia, or stem dendrites were lower in glutamate transporters than those facing nerve terminals, axons, and spines. Parallel fiber boutons (glutamatergic) synapsin on interneuron dendritic shafts were surrounded by lower transporter densities than those synapsing on Purkinje cell spines. Our findings suggest the localizations of glutamate transporters are carefully regulated.

References

Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·T StorckW Stoffel
Jul 1, 1992·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K D PhendA Rustioni
Sep 1, 1991·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M van Lookeren CampagneA J Verkleij
Nov 1, 1994·Progress in Neurobiology·N C Danbolt
Sep 1, 1994·Neuron·J D RothsteinR W Kuncl
Jun 30, 1994·Nature·D Attwell
Sep 1, 1993·Neuron·D AttwellM Szatkowski
Oct 1, 1995·The European Journal of Neuroscience·L M LevyN C Danbolt

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Citations

Jun 28, 2000·The Journal of Comparative Neurology·C A VandenbrandenM Kamermans
Apr 12, 2002·Journal of Neuroscience Research·Jens GroscheAndreas Reichenbach
Jul 12, 2002·Journal of Neuroscience Research·Jagdeep K SandhuP Roy Walker
Nov 1, 1996·Journal of Biomedical Science·D.L. Kolson, R.J. Pomerantz
Aug 29, 2001·Journal of Tongji Medical University = Tong Ji Yi Ke Da Xue Xue Bao·J ChenA Mendelowitsch
Jun 3, 2008·Cellular and Molecular Life Sciences : CMLS·A NairS D Miller
Feb 3, 2007·Pflügers Archiv : European journal of physiology·Sergei KirischukAlexei Verkhratsky
May 7, 2005·Neuroscience and Behavioral Physiology·A V Sem'yanov
Feb 1, 1996·Brain Research. Molecular Brain Research·M BjøråsN C Danbolt
Feb 7, 2013·Neurochemistry International·Susan C Buckingham, Stefanie Robel
May 3, 2005·Neuroscience Letters·Yoshitaka ShimizuKiyofumi Gyo
Aug 29, 2013·Progress in Neurobiology·Annalisa Buffo, Ferdinando Rossi
Apr 23, 2004·Trends in Neurosciences·Robert T FremeauRobert H Edwards
Dec 18, 2001·Brain Research. Developmental Brain Research·S FukamachiT Iwaki
Jun 25, 2002·Trends in Neurosciences·Gilles BonventoLuc Pellerin
Aug 11, 1999·Trends in Neurosciences·M A Bunin, R M Wightman
Apr 27, 2000·Trends in Neurosciences·M E Rice
Aug 15, 2002·Neurochemistry International·Susan G Amara, Andreia C K Fontana
Jul 6, 2000·Progress in Neurobiology·G Carmignoto
May 23, 2001·Progress in Neurobiology·N C Danbolt

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