Glutamate uptake in Lactobacillus delbrueckii subsp. bulgaricus CNRZ 208 and its enhancement by a combination of Mn2+ and Mg2+

Letters in Applied Microbiology
G Savoy de GioriD Hemme

Abstract

To demonstrate the mechanism of glutamate uptake in the dairy strain Lactobacillus delbrueckii subsp. bulgaricus CNRZ 208, and to characterize key aspects of the system. Glutamate uptake proceeded via an active transport system requiring an exogenous source of energy. The system also transported aspartate and glutamine. It was unique, with a Kt of 2.8 micro mol l-1 and a Vmax of 900 micro mol s-1 (g dry weight)-1. The activity was optimal at pH 7.3 and 50 degrees C, was independent of the glutamate charge, and was enhanced by Mn2+ + Mg2+ in combination. Inhibition of the activity by uncouplers and ionophores showed that transport was driven by an ATP-dependent mechanism involving the proton-motive force. This inhibition was partially abolished in the presence of both Mn2+ and Mg2+. We demonstrated for the first time that an active transport system governs the uptake of the essential amino acid glutamate in Lact. delbrueckii subsp. bulgaricus CNRZ 208, the activity of which is enhanced by a combination of Mn2+ and Mg2+. The potential of the findings is discussed with reference to the growth of Lact. delbrueckii subsp. bulgaricus in mixed-strain cultures for the dairy industry.

References

Jul 9, 2004·Applied and Environmental Microbiology·Makoto AshiuchiHaruo Misono
Aug 9, 2006·Critical Reviews in Microbiology·María Fernández, Manuel Zúñiga
May 29, 2018·Applied Microbiology and Biotechnology·Mamata SinghviKenji Sonomoto

Citations

Feb 1, 1977·The Journal of Applied Bacteriology·O V LedesmaJ V Galpin
Apr 1, 1984·Journal of Bacteriology·F S Archibald, M N Duong
Jan 1, 1994·Antonie van Leeuwenhoek·Wil N KoningsH W Van Veen
Jul 1, 1997·Perspectives in Biology and Medicine·E D Weinberg
May 3, 2000·Journal of Applied Microbiology·M ElliL Morelli
Feb 13, 2001·Letters in Applied Microbiology·C FoucaudM Desmazeaud
May 1, 1994·World Journal of Microbiology & Biotechnology·G Savoy de Giori, G F de Valdez

Related Concepts

Manganese
Aspartate
Uptake
Glucose, (beta-D)-Isomer
Anhydrous Dextrose
Uncouplers [MoA]
Ionophores
Metabolic Inhibition
Glutamic Acid, (D)-Isomer
Enzyme Inhibitors

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