Glutamatergic neurons induce expression of functional glutamatergic synapses in primary myotubes.

PloS One
Michele EttorreMario Buffelli

Abstract

The functioning of the nervous system depends upon the specificity of its synaptic contacts. The mechanisms triggering the expression of the appropriate receptors on postsynaptic membrane and the role of the presynaptic partner in the differentiation of postsynaptic structures are little known. To address these questions we cocultured murine primary muscle cells with several glutamatergic neurons, either cortical, cerebellar or hippocampal. Immunofluorescence and electrophysiology analyses revealed that functional excitatory synaptic contacts were formed between glutamatergic neurons and muscle cells. Moreover, immunoprecipitation and immunofluorescence experiments showed that typical anchoring proteins of central excitatory synapses coimmunoprecipitate and colocalize with rapsyn, the acetylcholine receptor anchoring protein at the neuromuscular junction. These results support an important role of the presynaptic partner in the induction and differentiation of the postsynaptic structures.

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Citations

Jul 3, 2017·Brain Structure & Function·Claudia LaperchiaMarina Bentivoglio

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Methods Mentioned

BETA
confocal microscopy
co-immunoprecipitation
coimmunoprecipitation

Software Mentioned

QED InVivo

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