Glutamic acid 160 is the acid-base catalyst of beta-xylosidase from Bacillus stearothermophilus T-6: a family 39 glycoside hydrolase

FEBS Letters
T BravmanY Shoham

Abstract

A beta-xylosidase from Bacillus stearothermophilus T-6 was cloned, overexpressed in Escherichia coli and purified to homogeneity. Based on sequence alignment, the enzyme belongs to family 39 glycoside hydrolases, which itself forms part of the wider GH-A clan. The conserved Glu160 was proposed as the acid-base catalyst. An E160A mutant was constructed and subjected to steady state and pre-steady state kinetic analysis together with azide rescue and pH activity profiles. The observed results support the assignment of Glu160 as the acid-base catalytic residue.

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Citations

Mar 13, 2002·Archives of Biochemistry and Biophysics·Tünde KissLászló Kiss
Jul 2, 2003·Current Opinion in Microbiology·Dalia Shallom, Yuval Shoham
Sep 21, 2012·Acta Crystallographica. Section D, Biological Crystallography·Camila Ramos SantosMario Tyago Murakami
Jul 19, 2012·World Journal of Microbiology & Biotechnology·Luciana GracianoRita de Cássia Garcia Simão
Dec 24, 2014·BMC Biotechnology·Aditya BhallaRajesh K Sani
Mar 17, 2007·Biochimica Et Biophysica Acta·Denny QuinteroLellys M Contreras
Oct 11, 2005·Journal of Molecular Biology·Mirjam CzjzekYuval Shoham
Feb 18, 2014·Acta Crystallographica. Section D, Biological Crystallography·Shifra LanskyGil Shoham
Jun 5, 2020·The Journal of Biological Chemistry·Smadar ShulamiYuval Shoham
Apr 5, 2014·Acta Crystallographica. Section F, Structural Biology Communications·Shifra LanskyGil Shoham
Aug 2, 2014·Acta Crystallographica. Section F, Structural Biology Communications·Shifra LanskyGil Shoham
Nov 6, 2014·Acta Crystallographica. Section D, Biological Crystallography·Shifra LanskyGil Shoham
Jun 6, 2020·Frontiers in Bioengineering and Biotechnology·Mariana Abrahão Bueno de MoraisMário Tyago Murakami
Dec 6, 2006·Archives of Biochemistry and Biophysics·Elena V EneyskayaAnna A Kulminskaya

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