PMID: 8613769Dec 1, 1995Paper

Glutaminase-positive and glutaminase-negative pyramidal cells in layer VI of the primary motor and somatosensory cortices: a combined analysis by intracellular staining and immunocytochemistry in the rat

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
T KanekoN Mizuno

Abstract

Pyramidal neurons in layer VI of the primary motor and somatosensory cortices were examined by a combined method of intracellular recording, biocytin injection, and immunocytochemistry using in vitro slice preparations of rat brain immunofluorescence staining revealed that biocytin-injected pyramidal cells in layer VI were separated into glutaminase (PAG)-immunopositive and PAG-immunonegative cells. Although the two groups of pyramidal cells showed no statistically significant differences in passive membrane properties and spike characteristics, a clear difference was found in spike afterpotentials. Ten of 12 PAG-positive pyramidal cells showed no or a small fast afterhyperpolarization (fAHP), whereas 10 of 11 PAG-negative pyramidal cells displayed a large fAHP. Depolarizing afterpotentials were observed only in PAG-positive pyramidal cells than in PAG-negative cells. In contrast, the arborization of basal dendrites was more developed in PAG-positive pyramidal cells than in PAG-negative cells. The main axons of all the pyramidal cells entered the subcortical axons of all the pyramidal cells entered the subcortical white matter. The local axon collaterals of PAG-positive pyramidal cells were widely spread in the horizontal direc...Continue Reading

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