Glutamine and glutamate limit the shortening of action potential duration in anoxia-challenged rabbit hearts

Physiological Reports
Kenneth J DrakeVeniamin Y Sidorov

Abstract

In clinical conditions, amino acid supplementation is applied to improve contractile function, minimize ischemia/reperfusion injury, and facilitate postoperative recovery. It has been shown that glutamine enhances myocardial ATP/APD (action potential duration) and glutathione/oxidized glutathione ratios, and can increase hexosamine biosynthesis pathway flux, which is believed to play a role in cardioprotection. Here, we studied the effect of glutamine and glutamate on electrical activity in Langendorff-perfused rabbit hearts. The hearts were supplied by Tyrode's media with or without 2.5 mmol/L glutamine and 150 μmol/L glutamate, and exposed to two 6-min anoxias with 20-min recovery in between. Change in APD was detected using a monophasic action potential probe. A nonlinear mixed-effects regression technique was used to evaluate the effect of amino acids on APD over the experiment. Typically, the dynamic of APD change encompasses three phases: short transient increase (more prominent in the first episode), slow decrease, and fast increase (starting with the beginning of recovery). The effect of both anoxic challenge and glutamine/glutamate was cumulative, being more pronounced in the second anoxia. The amino acids' protective ...Continue Reading

References

May 1, 1978·Circulation Research·E Carmeliet
Feb 1, 1992·Annals of Surgery·R W HongD W Wilmore
May 1, 1991·Progress in Cardiovascular Diseases·M R Franz
Feb 1, 1987·Circulation Research·D G Allen, C H Orchard
Jan 1, 1969·Quarterly Journal of Experimental Physiology and Cognate Medical Sciences·J H Ottaway
Apr 1, 1970·The Journal of Physiology·A S FreedbergE M Williams
Feb 1, 1994·Current Problems in Cardiology·H Taegtmeyer
Aug 4, 1997·The American Journal of Cardiology·H TaegtmeyerO H Frazier
Jan 25, 2000·American Journal of Physiology. Endocrinology and Metabolism·T E GrahamM J Gibala
Sep 21, 2000·American Journal of Physiology. Renal Physiology·L P Singh, E D Crook
Apr 12, 2001·International Journal of Experimental Pathology·B GuertlG Hoefler
Feb 15, 2002·Nutrition·Shihab E O KhogaliMichael J Rennie
Aug 29, 2003·American Journal of Physiology. Endocrinology and Metabolism·David M CohenHeinrich Taegtmeyer
Jan 30, 2004·Recent Progress in Hormone Research·Serafino FazioBernadette Biondi
Jun 11, 2005·Science·Michael E Mendelsohn, Richard H Karas
Dec 1, 2005·Science's STKE : Signal Transduction Knowledge Environment·Dona C Love, John A Hanover
Feb 14, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Micah S JohnsonDavid A Brown
Jun 20, 2006·Biochimica Et Biophysica Acta·Natasha E Zachara, Gerald W Hart
Aug 11, 2006·American Journal of Physiology. Cell Physiology·Voraratt ChampattanachaiJohn C Chatham
Sep 12, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Björn C KnollmannMichael R Franz
Nov 29, 2007·Asian Cardiovascular & Thoracic Annals·Gil BolotinValluvan Jeevanandam
Mar 28, 2008·American Journal of Physiology. Cell Physiology·Voraratt ChampattanachaiJohn C Chatham
Jul 22, 2008·Basic Research in Cardiology·Veniamin Y SidorovJohn P Wikswo
Dec 17, 2010·Circulation·Véronique L RogerUNKNOWN American Heart Association Statistics Committee and Stroke Statistics Subcommittee
Dec 15, 2011·The Heart Surgery Forum·Vladimir V LomivorotovAlexander M Karaskov
Jul 26, 2012·Experimental Diabetes Research·Rebecca Hutcheson, Petra Rocic
Dec 4, 2012·Journal of Molecular and Cellular Cardiology·Benjamin LauzierChristine Des Rosiers
Jan 29, 2013·Experimental Biology and Medicine·Kenneth J DrakeJohn P Wikswo
Jul 19, 2013·Biometrics·M S ShotwellJ P Wikswo

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Citations

Apr 23, 2021·Tissue Engineering and Regenerative Medicine·Thi-Tuong Van NguyenPhuc Van Pham

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Methods Mentioned

BETA
glycosylation

Software Mentioned

MATLAB

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