PMID: 7035832Jan 1, 1981Paper

Glutamine auxotrophs with mutations in a nitrogen regulatory gene, ntrC, that is near glnA

Molecular & General Genetics : MGG
G R Wei, S Kustu

Abstract

Some mutations to glutamine auxotrophy in the 86 unit region of the Salmonella chromosome lie within the nitrogen regulatory gene, ntrC, rather than the structural gene encoding glutamine synthetase, glnA, Assignment of mutations to ntrC is based on fine structure mapping by P22-mediated transduction and on complementation analysis. Strains with ntrC lesions that cause glutamine auxotrophy (NtrCrepressor) have very low levels of glutamine synthetase (lower than those of strains that completely lack ntrC function and comparable to those of strains that lack ntrA function). NtrCrep strains fail to increase synthesis of glutamine synthetase or several amino acid transport components under nitrogen limiting conditions. Thus, like ntrA strains, they appear to repress glnA transcription and fail to activate transcription of glnA or other nitrogen controlled genes. Mutations that suppress the glutamine requirement caused by NtrCrep lesions arise at high frequency; these mutations also suppress the glutamine requirement caused by ntrA lesions. Several suppressor mutations result in loss of function of ntrC.

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Citations

Jan 1, 1982·Molecular & General Genetics : MGG·T MacNeilB Tyler
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J HirschmanS Kustu
Oct 7, 2004·Applied and Environmental Microbiology·Luciano F HuergoFábio O Pedrosa
Sep 1, 1987·Molecular Microbiology·S A MacFarlane, M J Merrick
Sep 13, 2011·Biochimica Et Biophysica Acta·Nicolas JolyXiaodong Zhang
Jun 3, 1982·Nature·M J Merrick
Dec 1, 1995·Microbiological Reviews·M J Merrick, R A Edwards
Sep 1, 1983·Microbiological Reviews·K E Sanderson, J R Roth
Jan 1, 1993·Journal of Bacteriology·S Maloy, V Stewart
Dec 1, 1984·Journal of Bacteriology·G F AmesS Kustu
Dec 11, 1997·Journal of Bacteriology·L HeS Kustu
Sep 1, 1982·Journal of Bacteriology·D B JanssenC van der Drift

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