PMID: 29491345Mar 2, 2018Paper

Glutamine Enhances the Hypoglycemic Effect of Insulin in L6 Cells via Phosphatidylinositol-3-Kinase (PI3K)/Protein Kinase B (AKT)/Glucose Transporter 4 (GLUT4) Signaling Pathway

Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
Caijuan WangZhongming Wu

Abstract

BACKGROUND Diabetes mellitus (DM) is characterized by a decreased blood level of glutamine (Gln), which may contribute to the disturbance in the effect of insulin on skeletal muscle. Therefore, it is crucial to study how to improve the effect of insulin on skeletal muscle by increasing Gln. In the present study, we investigated the effect of Gln on the hypoglycemic action of insulin in skeletal muscle L6 cells at high glucose levels through the insulin signaling pathway and glycogen synthesis pathway. MATERIAL AND METHODS The L6 cells were cultured in and stimulated by Gln and insulin. The glutamine analogue, L-Gamma-Glutamyl-p-nitroanilide (GPNA), was used for verifying the effect of Gln. The expression of insulin signaling molecules, including phosphatidylinositol-3-kinase (PI3K), 3-phosphoinositide-dependent protein kinase-1 (PDK1), protein kinase B (AKT), protein kinase C zeta (PKCz), and glucose transporter 4 (GLUT4), were detected by real-time PCR and Western blot analysis, GLUT4 translocation was observed by immunofluorescence staining, glycogen synthase kinase (GSK) was analyzed by Western blotting, and glucose uptake was measured by glucose oxidase method (GOD). RESULTS The results demonstrated that Gln combined with i...Continue Reading

Citations

Nov 15, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ping ZhaoXinzhou Yang
Mar 20, 2019·Scientific Reports·Abdelilah ArredouaniMohammed Dehbi
Jan 10, 2020·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Yan FengJianwei Hu
Jan 25, 2020·Journal of Cellular Physiology·Yu FengRengfei Shi
Aug 24, 2019·Progress in Lipid Research·Tian-Tian ZhangChang-Hu Xue
Apr 22, 2021·BioMed Research International·Chunting LuZejian Li

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