Glutamine Modulates Changes in Intestinal Intraepithelial γδT-Lymphocyte Expressions in Mice With Ischemia/Reperfusion Injury

Shock
Man-Hui PaiChiu Li Yeh

Abstract

This study investigated the effect of glutamine (GLN) on expressions of small intestinal intraepithelial lymphocyte (IEL) γδT-cell proinflammatory cytokines and apoptotic regulatory factor genes in a mouse model of hindlimb ischemia/reperfusion (IR) injury. Mice were assigned to a normal control group and three IR groups. Mice in the normal control group received no ischemia treatment, whereas IR groups had hindlimb ischemia for 90 min with subsequent 0 (IR0) or 24 h (IR24) of reperfusion. The IR0 group was sacrificed immediately after reperfusion. The IR24S group was injected with saline, and the IR24G group was given 0.75 g GLN/kg of body weight once via a tail vein before reperfusion. The IR24 groups were sacrificed 24 h after reperfusion. Small intestinal IEL γδT cells of the animals were isolated for further analysis. Results showed that IR injury resulted in lower small intestinal IEL γδT-cell percentages and higher proinflammatory cytokine messenger RNA expressions of interleukin-1β (IL-1β), IL-6, and tumor necrosis factor-α by IEL γδT cells. Compared with the IR24S group, the IR24G group had a higher IEL γδT-cell percentage. Multiples of change of messenger RNA of proliferation gene expressions of the antiapoptotic Bcl-...Continue Reading

References

Jul 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·K MakiK Ikuta
Jan 1, 1996·JPEN. Journal of Parenteral and Enteral Nutrition·J M LaceyD W Wilmore
Jul 31, 1998·Nutrition·D W Wilmore, J K Shabert
Aug 5, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·A KhoshnanA E Nel
May 30, 2002·Nature Reviews. Immunology·Simon R Carding, Paul J Egan
Jul 11, 2002·Intensive Care Medicine·Rachid ZegdiAlain Tenaillon
Oct 12, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yaping ChenRichard Boismenu
Mar 26, 2003·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·W K EdreesC V Soong
May 14, 2005·Critical Care Medicine·Daurea A De-Souza, Lewis J Greene
Nov 23, 2005·Acta Cirúrgica Brasileira·Marcos Antonio AlvesPaulo Roberto Leitão de Vasconcelos
Dec 31, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jeffrey R ScottRichard F Potter
Dec 24, 2009·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Keisuke NoseDaniel H Teitelbaum
Mar 12, 2011·Naunyn-Schmiedeberg's Archives of Pharmacology·Emanuela EspositoSalvatore Cuzzocrea

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Citations

Jun 13, 2015·Shock·Mark G Clemens

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