Apr 1, 1984

Glutamine oxidation by dissociated cells and homogenates of rat brain: kinetics and inhibitor studies

Journal of Neurochemistry
J T Tildon, L M Roeder

Abstract

The rates of [U-14C]glutamine oxidation to 14CO2 were determined under a variety of experimental conditions using whole homogenates and dissociated cells from rat brain. The pattern of glutamine oxidation by homogenates differed from that by dissociated brain cells in several respects. The rates of glutamine oxidation by dissociated brain cells showed saturation kinetics with an apparent Km of 0.30 mM. Lineweaver-Burk plots of glutamine oxidation by homogenates revealed two linear segments with two apparent Km values (0.58 mM and 3.0 mM). In the presence of aminooxyacetate, however, the Lineweaver-Burk plots for homogenates were linear with a single Km of 0.47 mM. The oxidation of glutamine by homogenates was inhibited by both rotenone and antimycin A (80-85%), as were glutamate and glucose oxidation, suggesting that a significant amount of glutamine is oxidized via the tricarboxylic acid cycle. In the presence of aminooxyacetate, glutamine oxidation was inhibited less than 40%, whereas the oxidation of glutamate was inhibited 75%; in contrast, glucose oxidation was enhanced 50%. The rates of glutamine oxidation by homogenates were highest in the presence of high levels of potassium (50 mM) and low levels of sodium (2.5 mM). Va...Continue Reading

  • References33
  • Citations16

References

  • References33
  • Citations16

Mentioned in this Paper

August Rats
Rotenone
Brain
Antimycin A
Glutamate Decarboxylase
Glucose, (beta-D)-Isomer
Oxidation
Rat Brain
Brain Cell
SGOT - Glutamate oxaloacetate transaminase

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