Glutamine supplementation decreases intestinal permeability and preserves gut mucosa integrity in an experimental mouse model

JPEN. Journal of Parenteral and Enteral Nutrition
Rosana das Graças Carvalho dos SantosValbert Nascimento Cardoso

Abstract

Glutamine (GLN) is the preferred fuel for enterocytes, and GLN supplementation is critical during stressful conditions. The aim of this study was to evaluate the effect of GLN on intestinal barrier permeability and bacterial translocation in a murine experimental model. Swiss male mice (25-30 g) were randomized into 3 groups: (1) sham group; (2) intestinal obstruction (IO) group; (3) IO and GLN (500 mg/kg/d) group. Two different experiments were carried out to assess intestinal permeability and bacterial translocation. In the first experiment, the animals were divided into the 3 groups described above and received diethylenetriamine pentaacetate radiolabeled with technetium ((99m)Tc) on the eighth day. At different time points after intestinal obstruction, blood was collected to determine radioactivity. The animals were killed, and the small intestine was removed for histological analyses. In the bacterial translocation study, on the eighth day all groups received Escherichia coli labeled with (99m)Tc. After 90 minutes, the animals underwent intestinal obstruction and were killed 18 hours later. Blood, mesenteric lymph nodes, liver, spleen, and lungs were removed to determine radioactivity. Statistical significance was consider...Continue Reading

Associated Clinical Trials

References

Jun 22, 1999·Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine·S O DinizV N Cardoso
Feb 13, 2001·The Journal of Surgical Research·T ChangL Tsai
Aug 2, 2001·Journal of Pediatric Gastroenterology and Nutrition·S M Lichtman
Feb 8, 2002·World Journal of Gastroenterology : WJG·Jun-You LiYong-Ming Yao
May 3, 2002·Nutrition·Paolo R O Salvalaggio, Antonio C L Campos
May 24, 2003·Best Practice & Research. Clinical Gastroenterology·Reiner Wiest, Heiko C Rath
Jun 12, 2003·World Journal of Gastroenterology : WJG·Lian-An Ding, Jie-Show Li
Apr 20, 2004·Current Opinion in Clinical Nutrition and Metabolic Care·Gerdien C MelisPaul A M van Leeuwen
Jan 18, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Fourogh KatouzianAlessandro Ventura
Oct 11, 2005·Nutrition in Clinical Practice : Official Publication of the American Society for Parenteral and Enteral Nutrition·Paul E Wischmeyer
Oct 28, 2005·Advances in Surgery·Awori J HayangaGregory B Bulkley
Feb 9, 2006·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·J S WhiteT Diamond
Feb 14, 2006·Nutrition·Mariana Andrade de OliveiraValbert Nascimento Cardoso
Sep 13, 2006·Gut·M C ArrietaJ B Meddings
Feb 20, 2007·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Iara Eliza Pacífico QuirinoValbert Nascimento Cardoso

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Citations

Jul 14, 2012·HPB Surgery : a World Journal of Hepatic, Pancreatic and Biliary Surgery·Arash NickkholghPeter Schemmer
Jun 13, 2012·World Journal of Gastroenterology : WJG·Yaron Ilan
Jun 19, 2013·The British Journal of Nutrition·Rosana G C SantosValbert N Cardoso
Apr 12, 2014·Journal of Negative Results in Biomedicine·Tatiani Uceli MaioliSimone de Vasconcelos Generoso
Jan 26, 2016·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Hong-chang LiDian-xu Feng
Nov 21, 2012·International Journal of Surgery·Aslı ÇıtakFatma Yurt Lambrecht
Aug 26, 2014·The Journal of Surgical Research·Etem AlhanCengiz Erçin
Sep 18, 2013·JPEN. Journal of Parenteral and Enteral Nutrition·Joseph F PierreKenneth A Kudsk
Aug 24, 2012·JPEN. Journal of Parenteral and Enteral Nutrition·Iara Eliza Pacífico QuirinoMaria Isabel Toulson Davisson Correia
May 20, 2014·JPEN. Journal of Parenteral and Enteral Nutrition·Xinying WangKenneth A Kudsk
Aug 20, 2014·JPEN. Journal of Parenteral and Enteral Nutrition·Iara Eliza Pacífico QuirinoMaria Isabel Correia
Oct 25, 2011·JPEN. Journal of Parenteral and Enteral Nutrition·Marina Andrade BatistaValbert Nascimento Cardoso
Jan 13, 2015·JPEN. Journal of Parenteral and Enteral Nutrition·Maísa Mota AntunesMaria Isabel Toulson Davisson Correia
Oct 27, 2011·Yonsei Medical Journal·Hyeyoung Kim
Apr 23, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Joyce C McCannBruce N Ames
Nov 30, 2019·Experimental and Therapeutic Medicine·Huping HuangYali Zhang
Oct 28, 2019·International Journal of Molecular Sciences·Simone PernaMariangela Rondanelli
Dec 9, 2020·Journal of Applied Microbiology·Mayana Rodrigues S RibeiroMaria Aparecida Gomes
Oct 14, 2021·Journal of Neurogastroenterology and Motility·Young Min KimHyojin Park

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