Glutamine synthetase degradation is controlled by oxidative proteolysis in the marine cyanobacterium Prochlorococcus marinus strain PCC 9511

Biochimica Et Biophysica Acta
Guadalupe Gómez-BaenaJesús Diez

Abstract

Prochlorococcus is one of the most important primary producers on Earth; its unusual features and ecological importance have made it a model organism, but nutrient assimilation has received little attention. Glutamine synthetase (GS) plays a key role in nitrogen metabolism and its central position justifies the fine regulation of this enzyme. The aim of this work is to demonstrate the involvement of metal-catalyzed oxidation (MCO) in the control of the biological activity and turnover of GS from Prochlorococcus. In order to study the physiological role of MCO, we have first characterized the in vitro biosynthetic inactivation and degradation of GS in the axenic PCC 9511 strain, testing then the effect of several stress conditions, such as the presence of electron transport inhibitors, darkness and aging, on the inactivation and degradation of GS. It is noteworthy that the physiological substrates of GS could protect the enzyme from the oxidative inactivation and ATP partially reverted this inactivation once the enzyme had been oxidized, being this effect higher in the presence of glutamate. We have also found that the GS from aged cultures is degraded to the same smaller size fragments obtained in the in vitro degradation of GS...Continue Reading

Citations

Jul 26, 2014·PloS One·María Agustina Domínguez-MartínJosé Manuel García-Fernández
Feb 1, 2009·Environmental Microbiology Reports·Oriol Alberto RangelJose Manuel García-Fernández
Apr 1, 2012·Environmental Microbiology Reports·Brian McDonaghJose M García Fernández
Jul 23, 2016·Frontiers in Microbiology·María Agustina Domínguez-MartínJosé M García-Fernández
Feb 11, 2014·Journal of Bacteriology·Oxana S TarassovaGeorge W Owttrim
Dec 22, 2017·FEMS Microbiology Letters·María Agustina Domínguez-MartínJosé Manuel García-Fernández

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