Glutamine synthetase gene expression during the regeneration of the annelid Enchytraeus japonensis

Development Genes and Evolution
Cintia Carla NivaM Myohara

Abstract

Enchytraeus japonensis is a highly regenerative oligochaete annelid that can regenerate a complete individual from a small body fragment in 4-5 days. In our previous study, we performed complementary deoxyribonucleic acid subtraction cloning to isolate genes that are upregulated during E. japonensis regeneration and identified glutamine synthetase (gs) as one of the most abundantly expressed genes during this process. In the present study, we show that the full-length sequence of E. japonensis glutamine synthetase (EjGS), which is the first reported annelid glutamine synthetase, is highly similar to other known class II glutamine synthetases. EjGS shows a 61-71% overall amino acid sequence identity with its counterparts in various other animal species, including Drosophila and mouse. We performed detailed expression analysis by in situ hybridization and reveal that strong gs expression occurs in the blastemal regions of regenerating E. japonensis soon after amputation. gs expression was detectable at the cell layer covering the wound and was found to persist in the epidermal cells during the formation and elongation of the blastema. Furthermore, in the elongated blastema, gs expression was detectable also in the presumptive reg...Continue Reading

References

Jan 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·G PesoleC Saccone
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·Y KumadaY Tateno
Apr 1, 1993·Developmental Dynamics : an Official Publication of the American Association of Anatomists·L VardimonL E Fox
May 1, 1995·JPEN. Journal of Parenteral and Enteral Nutrition·J C RoigJ Neu
Aug 29, 1998·Journal of Neurochemistry·H TokiH Kiyama
May 25, 1999·JPEN. Journal of Parenteral and Enteral Nutrition·M D WeissJ Neu
Nov 2, 1999·Development, Growth & Differentiation·M MyoharaS Tochinai
Oct 25, 2001·Gene·C T SmarttB M Christensen
Jun 5, 2003·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·T P MommsenM E Elliott
Sep 15, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Maroko Myohara
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Peter W Reddien, Alejandro Sánchez Alvarado
Jun 25, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Akira TazakiMakoto Mochii
Apr 13, 2006·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Henny W M van StraatenTheodorus B M Hakvoort
May 26, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Maroko MyoharaJae Min Lee
Nov 23, 2006·The Journal of Comparative Neurology·Jeremy M SullivanBarbara S Beltz

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Citations

Jun 12, 2010·Biological Chemistry·Jong-Ho ChoiKwang-Hyun Baek
Aug 30, 2012·Journal of Cellular Biochemistry·Jong-Ho ChoiKwang-Hyun Baek
May 18, 2011·Glia·Daniel K Hartline
Aug 10, 2016·Current Opinion in Genetics & Development·B Duygu Özpolat, Alexandra E Bely
Mar 7, 2021·International Journal of Molecular Sciences·Kornélia BodóPéter Engelmann

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Datasets Mentioned

BETA
AB109095

Methods Mentioned

BETA
amputation
gene knockdown

Software Mentioned

BLASTP
ClustalW

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