PMID: 40960Nov 1, 1979

Glutamine synthetase mutations which affect expression of nitrogen fixation genes in Klebsiella pneumoniae

Journal of Bacteriology
F M AusubelA P Peskin


Previous studies have implicated glutamine synthetase (L-glutamate:ammonia ligase [adenosine diphosphate for-ing], EC as a major controlling element of the nitrogen fixation (nif) genes in Klebsiella pneumoniae. We report here the isolation of a new class of K. pneumoniae mutants which exhibit altered patterns of nif and hut (histidine utlization) regulation. The expression of nif in these mutants, which were isolated as Gln+ (glutamine nonrequiring) revertants of a particular glnA mutation, is extremely sensitive to ammonia repression. These mutants have a Nif- Hut- phenotype at external ammonia concentrations at which wild-type strains are Nif+ Hut+. On the other hand, these mutants can be fully derepressed for nif at very low ammonia concentrations. We adopted the nomenclature "GlnR- (Nif- Hut-)" to facilitate discussion of the phenotype of these mutant strains. The mutations in these strains which confer the GlnR- phenotype map at or near glnA, the structural gene for glutamine synthetase.

Related Concepts

Glutamate-Ammonia Ligase
Histidine Ammonia-Lyase
Genes, Regulator
Klebsiella rhinoscleromatis
Klebsiella Infections
Vanadium Nitrogenase
Glutamate Dehydrogenase

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