Glutamine utilization by Rhizobium etli

Molecular Plant-microbe Interactions : MPMI
Rosarita TatèEduardo J Patriarca

Abstract

We undertook the study of the use of glutamine (Gln) as the source of carbon and energy by Rhizobium etli. Tn5-induced mutagenesis allowed us to identify several genes required for Gln utilization, including those coding for two broad-range amino acid transporters and a glutamate dehydrogenase. The isolated mutants were characterized by the analysis of their capacity i) to grow on different media, ii) to transport Gln (uptake assays), and iii) to utilize Gln as the C energy source (CO2 production from Gln). We show that Gln is degraded through the citric acid cycle and that its utilization as the sole C source is related to a change in the bacterial cell shape (from bacillary to coccoid form) and a high susceptibility to a thiol oxidative insult. Both these data and the analysis of ntr-dependent promoters suggested that Gln-grown bacteria are under a condition of C starvation and N sufficiency, and as expected, the addition of glucose counteracted the morphological change and increased both the bacterial growth rate and their resistance to oxidative stress. Finally, a nodulation analysis indicates that the genes involved in Gln transport and degradation are dispensable for the bacterial ability to induce and invade developing n...Continue Reading

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Citations

Oct 20, 2011·Molecular Plant-microbe Interactions : MPMI·Rosarita TatéEduardo J Patriarca
Oct 23, 2004·Molecular Plant-microbe Interactions : MPMI·Simona FerraioliEduardo J Patriarca
Oct 10, 2007·PLoS Computational Biology·Osbaldo Resendis-AntonioBernhard Ø Palsson
Mar 5, 2013·Annual Review of Plant Biology·Michael Udvardi, Philip S Poole
Mar 8, 2014·Critical Reviews in Microbiology·Michael Frederick Dunn
Dec 5, 2014·Critical Reviews in Microbiology·Severin RonneauJean-Jacques Letesson
May 20, 2015·Journal of Proteomics·Mami NambuMitsuyoshi Ueda
Nov 19, 2017·Applied and Environmental Microbiology·Guirong TangLi Luo

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