Glutaraldehyde-polymerized hemoglobin and tempol (PolyHb-tempol) has superoxide dismutase activity that can attenuate oxidative stress on endothelial cells induced by superoxide anion

Artificial Cells, Nanomedicine, and Biotechnology
Mengdi WuKunping Yan

Abstract

A Tempol compound with an amine group (4-amino-2,2,6,6-tetramethylpiperidine-1-oxyl, NH2-Tempol) was cross-linked to hemoglobin in a one-step polymerization reaction to produce a novel hemoglobin-based oxygen carrier (HBOC) designated PolyHb-Tempol. The reaction parameters, including the reaction time, pH, temperature, and ratio of reactants, were optimized, and the physiochemical properties of the resulting product were characterized. PolyHb-Tempol didn't show any toxicity towards endothelial cells. Furthermore, from observations of cell morphology and viability, PolyHb-Tempol showed a significant ability to inhibit or eliminate oxidative stress induced by superoxide free radicals. These results suggest that PolyHb-Tempol may potentially be suitable as an HBOC.

References

Apr 11, 2002·Chemical Reviews·Peng George WangAdam J Janczuk
Mar 27, 2004·Nature Reviews. Drug Discovery·Abdu I Alayash
Jun 9, 2004·Free Radical Biology & Medicine·Paul W BuehlerCarleton J C Hsia
Aug 24, 2004·Artificial Organs·A Gerson Greenburg, Hae Won Kim
Jul 20, 2006·The Biochemical Journal·Jacqueline DunneChris E Cooper
May 27, 2009·British Journal of Pharmacology·Z LuoC S Wilcox
Aug 8, 2009·Antioxidants & Redox Signaling·Paul W Buehler, Felice D'Agnillo
Jan 25, 2013·Artificial Cells, Nanomedicine, and Biotechnology·Florina ScurtuRadu Silaghi-Dumitrescu
Mar 9, 2013·Artificial Cells, Nanomedicine, and Biotechnology·Mingzi LuHong Zhou
Nov 26, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Yon-Suk KimPyo-Jam Park
Mar 19, 2014·Trends in Biotechnology·Abdu I Alayash
Jun 26, 2014·Artificial Organs·Hans BäumlerRadostina Georgieva
Jan 20, 2016·Advances in Experimental Medicine and Biology·Khuanpiroon RatanasopaLeif Bülow

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