Glutathiolation of the proteasome is enhanced by proteolytic inhibitors

Archives of Biochemistry and Biophysics
M DemasiK J Davies

Abstract

The proteasome inhibitors lactacystin, clastro lactacystin beta-lactone, or tri-leucine vinyl sulfone (NLVS), in the presence of [(35)S]cysteine/methionine, caused increased incorporation of (35)S into cellular proteins, even when protein synthesis was inhibited by cycloheximide. This effect was blocked by incubation with the glutathione synthesis inhibitor buthionine sulfoximine. Proteasome inhibitors also enhanced total glutathione levels, increased reduced/oxidized glutathione ratio (GSH/GSSG) and upregulated gamma-glutamylcysteine synthetase (rate-limiting in glutathione synthesis). Micromolar concentrations of GSH, GSSG, or cysteine stimulated the chymotrypsin-like activity of purified 20S proteasome, but millimolar GSH or GSSG was inhibitory. Interestingly, GSH did not affect 20S proteasome's trypsin-like activity. Enhanced proteasome glutathiolation was verified when purified preparations of the 20S core enzyme complex were incubated with [(35)S]GSH after pre-incubation with any of the inhibitors. NLVS, lactacystin or clastro lactacystin beta-lactone may promote structural modification of the 20S core proteasome, with increased exposure of cysteine residues, which are prone to S-thiolation. Three main conclusions can be ...Continue Reading

References

Jan 1, 1995·Methods in Enzymology·A Meister
Jan 1, 1995·Methods in Enzymology·J A ThomasP Haddock
Apr 1, 1993·The Biochemical Journal·A J Rivett
May 10, 1995·Archives of Biochemistry and Biophysics·J A ThomasR Honzatko
Sep 1, 1994·Trends in Biochemical Sciences·J M Peters
Apr 30, 1996·Proceedings of the National Academy of Sciences of the United States of America·E Cabiscol, R L Levine
Jun 28, 1996·The Journal of Biological Chemistry·T GruneK J Davies
Jan 1, 1996·Annual Review of Biochemistry·O CouxA L Goldberg
Jan 3, 1997·The Journal of Biological Chemistry·L R DickR L Stein
Jun 24, 1997·Proceedings of the National Academy of Sciences of the United States of America·M BogyoH Ploegh
Aug 15, 1997·The Journal of Biological Chemistry·B S Berlett, E R Stadtman
Nov 14, 1997·The Journal of Biological Chemistry·J Jahngen-HodgeA Taylor
May 16, 1998·The Journal of Biological Chemistry·G Fenteany, S L Schreiber
May 12, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M ObinA Taylor
May 14, 1998·Molecular Aspects of Medicine·A TaylorM Obin
May 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·O UllrichK J Davies
Mar 29, 2000·Biochemical and Biophysical Research Communications·K R SekharM L Freeman
Aug 6, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·N SitteK J Davies

❮ Previous
Next ❯

Citations

Jun 25, 2009·The Journal of Biological Chemistry·Jaroslaw W ZmijewskiEdward Abraham
May 6, 2011·Molecular & Cellular Proteomics : MCP·Charity T AikenLan Huang
Jan 18, 2011·Antioxidants & Redox Signaling·Ying XiongDanyelle M Townsend
Feb 15, 2011·Antioxidants & Redox Signaling·Franziska KriegenburgRasmus Hartmann-Petersen
Jan 11, 2012·Antioxidants & Redox Signaling·Gustavo M SilvaMarilene Demasi
Mar 28, 2012·Cardiovascular Research·Marilene Demasi, Francisco R M Laurindo
Aug 2, 2005·Plant Physiology·David P DixonRobert Edwards
Aug 19, 2014·Antioxidants & Redox Signaling·Oliver Drews, Heinrich Taegtmeyer
Dec 14, 2004·FEBS Letters·Deborah A Ferrington, Rebecca J Kapphahn
Mar 1, 2011·Drug Metabolism Reviews·Kenneth D Tew, Danyelle M Townsend
Oct 7, 2014·Journal of Molecular and Cellular Cardiology·Marion MussbacherAstrid Schrammel
Jan 30, 2007·Experimental Eye Research·Rebecca J KapphahnDeborah A Ferrington
Jul 17, 2004·Journal of Cellular and Molecular Medicine·Daniela GiustariniIsabella Dalle-Donne
Feb 1, 2005·Biochimica Et Biophysica Acta·Isabelle Petropoulos, Bertrand Friguet
Apr 21, 2016·Archives of Biochemistry and Biophysics·Kelvin J A Davies
Dec 22, 2014·Molecular and Cellular Endocrinology·Jerry Vriend, Russel J Reiter
Oct 17, 2012·Biochimica Et Biophysica Acta·José Luis García-GiménezFederico V Pallardó
Sep 25, 2012·Free Radical Biology & Medicine·Kalavathi DasuriJeffrey N Keller
Apr 10, 2010·Current Gerontology and Geriatrics Research·A PetersenM Zetterberg
May 14, 2011·Free Radical Biology & Medicine·Rodney P Guttmann, Sarbani Ghoshal
Dec 3, 2013·Free Radical Biology & Medicine·Francisco H Dal VechioMarilene Demasi
Jan 8, 2014·Redox Biology·Marilene DemasiErina Ohara
Jul 15, 2016·Antioxidants & Redox Signaling·Annika HöhnTobias Jung
Apr 15, 2004·Archives of Biochemistry and Biophysics·Gisele MonteiroLuis E S Netto
Apr 28, 2018·Circulation Research·Steven J ForresterKathy K Griendling
Feb 1, 2007·American Journal of Physiology. Endocrinology and Metabolism·Kajorn KitiphongspattanaH Rex Gaskins
Feb 2, 2019·Current Pharmaceutical Design·Gulce SariBetul Karademir
Jun 1, 2006·Current Protocols in Toxicology·Aleksandra Filipovska, Michael P Murphy
Jun 26, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Christopher C FranklinNelson Fausto
Apr 21, 2020·Free Radical Research·Takujiro Homma, Junichi Fujii
Aug 14, 2019·International Journal of Molecular Sciences·Elise LévyLaurence Vernis
Apr 2, 2002·The Journal of Biological Chemistry·Philip EatonMichael J Shattock
Nov 1, 2002·The Journal of Biological Chemistry·Marilene DemasiLuis Eduardo Soares Netto
Aug 6, 2019·Frontiers in Molecular Biosciences·Suzan KorsSabine Schipper-Krom
Dec 10, 2020·Polymers·Lucia Račková, Erika Csekes
Jul 28, 2005·Molecular Cancer Research : MCR·Milena PervanWilliam H McBride
Aug 20, 2019·Biochimica Et Biophysica Acta. Molecular Cell Research·Senem SalcanSebastian Kötter
Aug 2, 2017·Redox Biology·Ioanna KorovilaTobias Jung
Jul 23, 2003·Journal of the American Society of Nephrology : JASN·Philip EatonMichael J Shattock

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.