PMID: 6991915May 1, 1980Paper

Glutathione and glutathione S-transferases in the Salmonella mammalian-microsome mutagenicity test

Mutation Research
K H SummerH Greim

Abstract

Levels of the tripeptide glutathione (GSH) and the activity of glutathione S-transferases were investigated in S9 fractions of rats and mice and in Salmonella typhymurium tester strains TA1535, TA100, TA1538 and TA98. The S9 and Salmonella typhimurium tester strains had high levels of glutathione. Compared with S9, the activity of GSH S-transferases was lower in the bacteria. However, electrophiles such as 1-chloro-2,4-dinitrobenzene (CDNB), diethyl maleate and styrene oxide were effectively bound to bacterial GSH. The mutagenicity of the direct mutagen CDNB was drastically lowered in presence of S9 fractions but not in presence of microsomes. A comparable decrease was obtained when microsomal supernatant, which contains GSH and GSH S-transferases, was added to the microsomes. Addition of GSH in excess completely abolished mutagenicity of CDNB. These results demonstrate that the conjugation of electrophiles with GSH mediated by the S9 fraction or the bacterial tester strains represents an important detoxication mechanism which may influence the results obtained with the Salmonella typhimurium mammalian-microsome mutagenicity test.

References

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Citations

Nov 1, 1980·Archives of Toxicology·H GreimT Wolff
Nov 1, 1989·Journal of Molecular Evolution·A R Sundquist, R C Fahey
Apr 1, 1980·Mutation Research·K H Summer, W Göggelmann
Dec 1, 1981·Mutation Research·C de MeesterF Poncelet
Jan 1, 1989·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·A G Clark
Jan 1, 1988·Toxicology in Vitro : an International Journal Published in Association with BIBRA·I M BruggemanP J van Bladeren
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·R ThierF P Guengerich
Jan 1, 1988·Antonie van Leeuwenhoek·E CasaloneM Polsinelli
Jan 1, 1987·Chemico-biological Interactions·J E SinsheimerV G Beylin
Mar 1, 1985·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·G L Larsen
Mar 1, 1983·Biochemistry·T J MozerE G Jaworski
Jun 15, 2014·Archives of Biochemistry and Biophysics·Hilary GroomP David Josephy
Jan 1, 1992·Environmental and Molecular Mutagenesis·D DillonE Zeiger
May 1, 1985·Journal of Bacteriology·D Kohler-Staub, T Leisinger

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