Glutathione and trypanothione in several strains of Trypanosoma cruzi: effect of drugs

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
Y RepettoA Morello

Abstract

Glutathione (GSH), trypanothione (T(SH)2) and glutathionyl spermidine (GSH-SP) concentrations were determined in the Tulahuén and LQ strains and the DM 28c clone of Trypanosoma cruzi. The concentrations of GSH, T(SH)2 and GSH-SP, expressed as nmol of GSH per g of parasite fresh weight, were 60.1, 397.8 and 103.9, respectively, for the Tulahuén strain. For the DM 28c clone, the values were 113.9, 677.9 and 164.1, respectively, and for the LQ strain they were 199.1, 1100.5 and 55.3, respectively. When the parasites were treated with 10 microM nifurtimox or 50 microM benznidazole for 2 h, the concentrations of all three reduced thiols decreased strongly. The total amount of T(SH)2 decreased by more than 50%. Treatment of the parasites with 5 mM buthionine sulfoximine, an inhibitor of GSH synthesis, for 6 h diminished the concentrations of the reduced thiols by between 27% and 53% with respect to the controls. Cyclohexylamine, an inhibitor of spermidine synthesis, decreased the concentrations of T(SH)2 and GSH-SP but not that of GSH. It is possible to conclude from this study that trypanothione is the most important thiol involved in the detoxication of nifurtimox and benznidazole in T. cruzi and that electrophilic reduced metaboli...Continue Reading

References

Oct 1, 1979·Archives of Biochemistry and Biophysics·R Docampo, A O Stoppani
Jan 1, 1992·Annual Review of Microbiology·A H Fairlamb, A Cerami
Jan 1, 1991·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·M H TsuhakoO Augusto
Oct 1, 1990·Biochemical Society Transactions·A H Fairlamb
Jan 1, 1989·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·C MoncadaA Morello
Jan 1, 1987·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·Y RepettoA Morello
Jan 1, 1988·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·A Morello
Jan 1, 1987·Transactions of the Royal Society of Tropical Medicine and Hygiene·L S Filardi, Z Brener
Dec 1, 1986·Molecular and Biochemical Parasitology·A H FairlambA Cerami
Apr 1, 1984·Biochemical Pharmacology·M MasanaJ A Castro
Apr 29, 1983·Science·A Meister
Jun 15, 1980·The Biochemical Journal·A BoverisA O Stoppani
Mar 1, 1981·The Journal of Experimental Medicine·B A ArrickA Cerami

❮ Previous
Next ❯

Citations

Nov 12, 2005·Parasitology Research·D MenezesM A Vannier-Santos
Mar 8, 2003·Biochemical Pharmacology·Juan Diego MayaGérard Chauvière
Nov 5, 1998·Biochemical Pharmacology·F E AranaF Gamarro
Apr 6, 1999·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·P RivasY Repetto
Mar 31, 2012·Antioxidants & Redox Signaling·Lucía PiacenzaRafael Radi
Dec 24, 2004·Antimicrobial Agents and Chemotherapy·Mario FaundezJuan Diego Maya
May 24, 2014·PLoS Neglected Tropical Diseases·Andrea TrochineCarlos Robello
Aug 4, 2010·Parasitology Research·Monica C O CamposSolange L de Castro
Oct 19, 2011·Journal of Parasitology Research·Marcia Cristina PaesJosé Roberto Meyer-Fernandes
Jun 13, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Catherine RoullierJoël Boustie
May 10, 2012·Journal of Chemical Ecology·Sven RohdeMarc Slattery
Apr 29, 2014·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Rajesh P Rastogi, Aran Incharoensakdi
Jul 13, 2005·The Biochemical Journal·Mark R AriyanayagamAlan H Fairlamb
Apr 28, 2018·Current Medicinal Chemistry·Stephen Patterson, Alan H Fairlamb
Sep 28, 2018·Current Medicinal Chemistry·Alan TaleviMarcelo Comini
Feb 26, 2005·Journal of Natural Products·Christine E Hand, John F Honek
Jun 8, 2007·Microbiology and Molecular Biology Reviews : MMBR·Michael W TaylorMichael Wagner

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.