Glutathione content of V79 cells in two- or three-dimensional culture

The American Journal of Physiology
F J RomeroW Mueller-Klieser

Abstract

The cellular glutathione (GSH) content of two- and three-dimensional cell cultures of V79 hamster lung cells has been studied. As previously described, cells in monolayer cultures show a decrease in GSH when they reach the confluent state. Three-dimensional cell cultures (multicell spheroids) allow a smoother transition from the initial proliferating to the nonproliferating status, and they show a central area of necrosis when a certain diameter is reached. Cellular GSH content in spheroids is variable throughout the culturing period: 1) GSH content (expressed per mg protein) is lower in spheroids with central necrotic areas than in smaller spheroids without necrosis, and 2) results expressed per cell number show a sharp increase around the diameter where necrosis appears. Once a relatively large necrotic area has been established, GSH decreases again to approximately the prenecrotic level. Interestingly, this GSH "peak" is not dependent on the time in culture but on the spheroid size. Acute hypoxia occurs in central areas of spheroids at a much higher size range than those described herein. Thus we suggest a combination of factors, which may include oxidative stress among others, as the explanation for these cellular GSH varia...Continue Reading

References

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Citations

Oct 28, 1998·Environmental Health Perspectives·F J RomeroJ Romá
Sep 13, 2002·American Journal of Physiology. Cell Physiology·Leoni A Kunz-Schughart, James P Freyer
Sep 13, 2007·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Xuehong Liu
Nov 14, 1997·The American Journal of Physiology·W Mueller-Klieser
Jun 6, 1998·International Journal of Experimental Pathology·L A Kunz-SchughartR Knuechel
Feb 14, 2009·Nature Protocols·Juergen FriedrichLeoni A Kunz-Schughart
Nov 2, 2019·Scientific Reports·Nina SchömelMarthe-Susanna Wegner
Oct 18, 2000·Critical Reviews in Oncology/hematology·W Mueller-Klieser
Aug 10, 2000·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·C JanzowskiG Eisenbrand
Mar 16, 2012·Journal of Proteome Research·K M McMahonC W Sutton

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