Glutathione disulfide production during arachidonic acid oxygenation in human platelets

Prostaglandins
J W Burch, P T Burch

Abstract

Washed human platelets stimulated with 50 microM sodium arachidonate rapidly accumulated glutathione disulfide to a peak concentration of 0.620 nmole per 10(9) cells, 200% of control (unstimulated) levels. Total glutathione remained unchanged. The rise in glutathione disulfide was transitory, returning to control values within 30 seconds in aggregating platelets. Similar findings were observed in washed platelets aggregated with 5 U/ml thrombin. Platelet aggregation was not necessary for the generation of glutathione disulfide. However, cyclooxygenase activity was necessary for the generation of glutathione disulfide. Aspirin treated platelets aggregated with thrombin demonstrated no thromboxane B2 production and no glutathione disulfide generation. Dose response studies with both agonists demonstrated a direct relationship between the amount of thromboxane B2 produced and the amount of glutathione disulfide generated by stimulated platelets. During the conversion of arachidonic acid to thromboxane B2, unesterified arachidonic acid is oxygenated to prostaglandin G2 which is subsequently reduced to prostaglandin H2. Both reactions are catalyzed by the enzyme prostaglandin H synthase. Our data support the hypothesis that glutathi...Continue Reading

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Citations

Dec 17, 1997·Clinical Biochemistry·L Mazzanti, B Mutus
Dec 14, 2011·Free Radical Biology & Medicine·Peter R Kvietys, D Neil Granger
Apr 16, 2011·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Maria I C Gyongyossy-Issa
Sep 16, 2009·Free Radical Biology & Medicine·Jane A Leopold, Joseph Loscalzo
Jul 23, 2003·Journal of Thrombosis and Haemostasis : JTH·J E Freedman, J Loscalzo
Sep 24, 2004·Physiological Reviews·Roland Stocker, John F Keaney
Jan 5, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Jane E Freedman
Jul 28, 2020·Antioxidants·Francesca SilvagnoGian Piero Pescarmona
May 20, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Rita Loch CarusoJames E Trosko

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