Glutathione import in Haemophilus influenzae Rd is primed by the periplasmic heme-binding protein HbpA.

Proceedings of the National Academy of Sciences of the United States of America
Bjorn VergauwenSavvas N Savvides

Abstract

Glutathione (GSH) is a vital intracellular cysteine-containing tripeptide across all kingdoms of life and assumes a plethora of cellular roles. Such pleiotropic behavior relies on a finely tuned spatiotemporal distribution of glutathione and its conjugates, which is not only controlled by synthesis and breakdown, but also by transport. Here, we show that import of glutathione in the obligate human pathogen Haemophilus influenzae, a glutathione auxotrophe, is mediated by the ATP-binding cassette (ABC)-like dipeptide transporter DppBCDF, which is primed for glutathione transport by a dedicated periplasmic-binding protein (PBP). We have identified the periplasmic lipoprotein HbpA, a protein hitherto implicated in heme acquisition, as the cognate PBP that specifically binds reduced (GSH) and oxidized glutathione (GSSG) forms of glutathione with physiologically relevant affinity, while it exhibits marginal binding to hemin. Dissection of the ligand preferences of HbpA showed that HbpA does not recognize bulky glutathione S conjugates or glutathione derivatives with C-terminal modifications, consistent with the need for selective import of useful forms of glutathione and the concomitant exclusion of potentially toxic glutathione addu...Continue Reading

References

Jul 14, 1975·Biochimica Et Biophysica Acta·P Apontoweil, W Berends
Jan 1, 1983·Annual Review of Biochemistry·A Meister, M E Anderson
Nov 1, 1995·Protein Science : a Publication of the Protein Society·P Dunten, S L Mowbray
Mar 27, 1998·Journal of Bacteriology·C Sherrill, R C Fahey
May 2, 2000·The Journal of Biological Chemistry·A BourboulouxA K Bachhawat
May 11, 2000·Proceedings of the National Academy of Sciences of the United States of America·Z Z ShiM W Lieberman
Jan 9, 2001·Archives of Biochemistry and Biophysics·J W PayneG M Payne
Feb 23, 2002·Molecular Plant-microbe Interactions : MPMI·R A CarterA W B Johnston
Feb 20, 2003·Journal of Bacteriology·Bjorn VergauwenJozef J Van Beeumen
Jan 1, 2004·The Journal of Biological Chemistry·Frederik PauwelsJozef J Van Beeumen
Dec 14, 2004·Journal of Molecular Biology·Vladimir M LevdikovAnthony J Wilkinson
Jul 26, 2005·The Journal of Biological Chemistry·Marc S PittmanRobert K Poole
Nov 18, 2005·FEMS Microbiology Letters·Daniel J MortonTerrence L Stull
Aug 15, 2006·Proceedings of the National Academy of Sciences of the United States of America·Sylvie LétofféCécile Wandersman
Jan 24, 2008·Infection and Immunity·Charles V RosadiniBrian J Akerley
Jun 7, 2008·Microbiology and Molecular Biology Reviews : MMBR·Amy L DavidsonJue Chen
Jul 22, 2008·Journal of Molecular Biology·Donald S BerkholzP Andrew Karplus
Jul 30, 2008·FEMS Yeast Research·Anil ThakurAnand K Bachhawat
Aug 23, 2008·Archives of Microbiology·Todd JohnsonMamta Rawat
Jan 10, 2009·The Journal of Allergy and Clinical Immunology·Anne M FitzpatrickUNKNOWN Severe Asthma Research Program
Jan 13, 2009·Trends in Biochemical Sciences·Isabella Dalle-DonneAldo Milzani
Mar 21, 2009·The EMBO Journal·Ronnie P-A BerntssonDirk-Jan Slotboom
May 20, 2009·International Journal of Medical Microbiology : IJMM·Daniel J MortonTerrence L Stull

❮ Previous
Next ❯

Citations

Feb 6, 2013·Infection and Immunity·Alistair HarrisonKevin M Mason
Nov 18, 2011·BMC Biochemistry·Bjorn VergauwenSavvas N Savvides
Apr 5, 2014·Current Opinion in Infectious Diseases·Farshid Jalalvand, Kristian Riesbeck
May 28, 2014·International Journal of Medical Microbiology : IJMM·Tamim Al JubairKristian Riesbeck
Feb 23, 2011·Current Opinion in Chemical Biology·Jeffery A MayfieldJennifer L DuBois
Dec 5, 2012·Biochimica Et Biophysica Acta·Anand K BachhawatM Zulkifli
Oct 19, 2012·Biochimica Et Biophysica Acta·Robert C Fahey
Feb 1, 2011·Current Opinion in Chemical Biology·Volkmar Braun, Klaus Hantke
Apr 9, 2015·Frontiers in Microbiology·Vu Van LoiHaike Antelmann
May 15, 2016·Free Radical Biology & Medicine·Natalia SasoniDiego G Arias
Nov 15, 2016·The Journal of Veterinary Medical Science·Yanhe ZhangChunlai Wang
Oct 14, 2018·The Journal of Biological Chemistry·Thanuja Gangi SettyRamaswamy Subramanian
Jun 13, 2012·Chemistry : a European Journal·Jeung-Hoi Ha, Stewart N Loh
Jul 12, 2017·BioMed Research International·Zhongshan WangQuanju Xiang
Dec 6, 2018·BioMed Research International·Zhongshan WangMeng Zhang
Aug 30, 2019·Free Radical Biology & Medicine·Natalia SasoniDiego G Arias
Nov 5, 2019·Research in Microbiology·Philippe Delepelaire

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Transport Proteins (ASM)

Bacterial transport proteins facilitate active and passive transport of small molecules and solutes across the bacterial membrane. Here is the latest research.

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.

Bacterial Transport Proteins

Bacterial transport proteins facilitate active and passive transport of small molecules and solutes across the bacterial membrane. Here is the latest research.