PMID: 9433907Jan 20, 1998Paper

Glutathione is a cofactor for H2O2-mediated stimulation of Ca2+-induced Ca2+ release in cardiac myocytes

Free Radical Biology & Medicine
Y J SuzukiM Morad

Abstract

Reactive oxygen species are known to cause attenuation of cardiac muscle contraction. This attenuation is usually preceded by transient augmentation of twitch amplitude as well as cytosolic Ca2+. The present study examines the role of an endogenous antioxidant, glutathione in the mechanism of H2O2-mediated augmentation of Ca2+ release from the sarcoplasmic reticulum. Whole-cell patch-clamped single rat ventricular myocytes were dialyzed with the Cs+-rich internal solution containing 200 microM fura-2 and 2 mM glutathione (reduced form). After equilibration of the myocyte with intracellular dialyzing solution, Ca2+ current-induced Ca2+ release from the sarcoplasmic reticulum was monitored. Rapid perfusion with H2O2 (100 microM or 1 mM) for 20 s inhibited Ca2+ current, but enhanced the intracellular Ca2+ transients for 3-4 min. Thus, the efficacy of Ca2+-induced Ca2+ release mechanism was augmented in 71% of myocytes (n = 7). This enhancement ranged between 1.5- to threefold as the concentrations of H2O2 were raised from 100 microM to 1 mM. If glutathione were excluded from the patch pipette or replaced with glutathione disulfide, the enhancement of Ca2+-induced Ca2+ release was seen in only a minority (20%) of the myocytes. H2O2...Continue Reading

References

Jul 1, 1992·Hypertension·J I Goldhaber, J N Weiss
Sep 1, 1990·Journal of Molecular and Cellular Cardiology·K P BurtonH K Hagler
Jan 1, 1988·Free Radical Biology & Medicine·R A Kloner
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Jul 1, 1983·The American Journal of Physiology·A Fabiato
Jan 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·J S ShamM Morad
Nov 1, 1996·The Journal of General Physiology·S Adachi-AkahaneM Morad
Jan 1, 1997·Free Radical Biology & Medicine·Y J SuzukiA Sevanian
May 7, 1954·Science·R GERSCHMANW O FENN

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Citations

Jul 15, 2004·Toxicology in Vitro : an International Journal Published in Association with BIBRA·She-Ching Wu, Gow-Chin Yen
Jun 18, 2002·European Journal of Pharmacology·Sefa ResimAtilla Dikmen
Jul 16, 2003·FEBS Letters·Aleksey V ZimaLothar A Blatter
Oct 5, 2001·Free Radical Biology & Medicine·K KittaY J Suzuki
Mar 10, 2001·Antioxidants & Redox Signaling·M MoradE Okabe
Mar 10, 2001·Antioxidants & Redox Signaling·I N Pessah, W Feng
Mar 10, 2001·Antioxidants & Redox Signaling·J I Goldhaber, M S Qayyum
Mar 10, 2001·Antioxidants & Redox Signaling·M Morad, Y J Suzuki
Apr 2, 2008·Antioxidants & Redox Signaling·Cecilia Hidalgo, Paulina Donoso
Nov 26, 2010·Antioxidants & Redox Signaling·Belma Turan, Guy Vassort
Jan 18, 2011·Antioxidants & Redox Signaling·Jon M Fukuto, Samantha J Carrington
Jun 22, 2006·Acta Pharmacologica Sinica·Yuan YanJie Liu
Apr 24, 2014·Pflügers Archiv : European journal of physiology·Sang Woong ParkHana Cho
Aug 31, 2010·Seminars in Immunopathology·Grace Y LamJohn H Brumell
Aug 31, 2010·Respiratory Physiology & Neurobiology·Larissa A Shimoda, Clark Undem
Jul 23, 2005·IUBMB Life·Cecilia HidalgoM Angélica Carrasco
Aug 16, 2003·The Journal of Biological Chemistry·Paula AracenaCecilia Hidalgo
Jul 24, 2008·Dose-response : a Publication of International Hormesis Society·Regina M Day, Yuichiro J Suzuki
Jan 24, 2007·Physiological Reviews·Karen Bedard, Karl-Heinz Krause
Feb 3, 2012·Physiological Reviews·J T SylvesterJeremy P T Ward
Dec 7, 2002·The Journal of Biological Chemistry·Kazumi KittaYuichiro J Suzuki
Aug 16, 2008·Experimental Biology and Medicine·Kathryn M Jaques-RobinsonGary F Merrill
Sep 20, 2000·American Journal of Physiology. Heart and Circulatory Physiology·J GuoC A Ward

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