Glutathione modulation influences methyl mercury induced neurotoxicity in primary cell cultures of neurons and astrocytes

Neurotoxicology
Parvinder KaurTore Syversen

Abstract

Methyl mercury (MeHg) is highly neurotoxic and may lead to numerous neurodegenerative disorders. In this study, we investigated the role of glutathione (GSH) and reactive oxygen species (ROS) in MeHg-induced neurotoxicity, using primary cell cultures of cerebellar neurons and astrocytes. To evaluate the effect of GSH on MeHg-induced cytotoxicity, ROS and GSH were measured using the fluorescent indicators chloro methyl derivative of di-chloro di-hydro fluorescein diacetate (CMH(2)DCFDA) and monochlorobimane (MCB). Cell-associated MeHg was measured with (14)C-radiolabeled MeHg. Mitochondrial dehydrogenase activity was detected by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide]. MTT timeline study was also performed to evaluate the effects of both the concentration and duration of MeHg exposure. The intracellular GSH content was modified by pretreatment with N-acetyl cysteine (NAC) or di-ethyl maleate (DEM) for 12 h. Treatment with 5 microM MeHg for 30 min led to significant (p<0.05) increase in ROS and reduction (p<0.001) in GSH content. Depletion of intracellular GSH by DEM further increased the generation of MeHg-induced ROS in both cell cultures. Conversely, NAC supplementation increased intracellular GSH an...Continue Reading

References

Jan 1, 1991·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·T Sarafian, M A Verity
Jul 1, 1988·European Journal of Cancer & Clinical Oncology·J CarmichaelJ B Mitchell
Sep 1, 1985·Archives of Toxicology·L MagosW R Skipp
Jul 20, 1973·Science·F BakirR A Doherty
Oct 1, 1982·Journal of Toxicology. Clinical Toxicology·S B Elhassani
Sep 1, 1994·Environmental Health Perspectives·K MiuraN Imura
Nov 1, 1993·Journal of Neurochemistry·J I SagaraS Bannai
Dec 1, 1996·Toxicology and Applied Pharmacology·B H ChoiM Robles
Apr 1, 1998·Environmental Health Perspectives·N BallatoriW Wang
Jul 6, 2000·Progress in Neurobiology·R Dringen
Feb 9, 2002·Environmental Health Perspectives·Thomas W Clarkson
Feb 8, 2003·Brain Research. Molecular Brain Research·Gouri Shanker, Michael Aschner
Mar 5, 2003·Cellular and Molecular Life Sciences : CMLS·M ZafarullahM Ahmad
Oct 31, 2003·The New England Journal of Medicine·Thomas W ClarksonGary J Myers
Sep 1, 2004·Brain Research. Molecular Brain Research·Gouri ShankerMichael Aschner
Jun 17, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Tora Sund MorkenTore Syversen

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Citations

Apr 24, 2007·Neurotoxicity Research·Carolina JohanssonSandra Ceccatelli
Nov 26, 2011·Biological Trace Element Research·Haibo YangBin Xu
Aug 5, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Helena T HogbergAnna K Bal-Price
Apr 9, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Cecon T MahapatraMatthew D Rand
Nov 13, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Yubin ZhangDavid A Lawrence
Oct 31, 2008·Environmental Health : a Global Access Science Source·Jean-Paul BourdineaudMuriel Laclau
May 22, 2013·TheScientificWorldJournal·Margaret E Sears
Jun 15, 2014·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Elsa GouixLaurence Had-Aissouni
Jun 21, 2013·Organic & Biomolecular Chemistry·Biswonath Biswal, Bamaprasad Bag
Dec 23, 2015·International Journal of Molecular Sciences·Arif Tasleem JanQazi Mohd Rizwanul Haq
Jun 5, 2012·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Tore Syversen, Parvinder Kaur
May 24, 2011·Toxicology and Applied Pharmacology·Marcelo FarinaJoão B T Rocha
Dec 15, 2010·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Yong LongZongbin Cui
Dec 21, 2010·Plant Physiology and Biochemistry : PPB·Mukesh K Yadav, Satish C Bhatla
Jun 30, 2009·Brain Research Bulletin·M G Zurich, F Monnet-Tschudi
May 5, 2009·Neurotoxicology·Matthew D RandTodd A Clason
May 20, 2009·Free Radical Biology & Medicine·Jeferson L FrancoMarcelo Farina
Jan 27, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Parvinder KaurTore Syversen
Oct 28, 2008·Chemosphere·Aaron M Shapiro, Hing Man Chan
Sep 24, 2011·Journal of Toxicology·Parvinder KaurTore Syversen
Jan 1, 2009·Environmental Toxicology and Pharmacology·Seunghwan LeeEuikyung Kim
Mar 18, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Parvinder KaurTore Syversen

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