PMID: 6245716Apr 17, 1980

Glutathione reduces cytoplasmic vanadate. Mechanism and physiological implications

Biochimica Et Biophysica Acta
I G MacaraL C Cantley

Abstract

The mechanism by which cells reduce cytoplasmic vanadium(V) (vanadate) to vanadium(IV) was investigated using the human red cell as a model system. Vanadate uptake by red cells occurs with a rapid phase involving chemical equilibration across the plasma membrane and a slower phase resulting in a high concentration of bound vanadium(IV). The slow phase was inhibited in glucose-starved cells and restored upon addition of glucose indicating an energy requirement for this process. The time course of vanadium(IV) appearance (monitored by EPR spectroscopy of intact cells) paralleled the slow phase of uptake indicating that this phase involves vanadium reduction. The reduction of intracellular vanadate to vanadium(IV) was nearly quantitative after 23 h. The intracellular reduction is not enzymatic, since a similar time course of vanadium reduction and binding to hemoglobin was observed when glutathione was added to a hemoglobin + vanadate solution in vitro. Vanadium(IV) binding to hemoglobin was reduced by addition of ATP, 2,3-diphosphoglycerate or EDTA, probably through chelation of the cation. The stability constant of the ATP-vanadium (IV) complex was determined to be 150 M-1 at pH 4.9. The time course of red cell vanadate uptake a...Continue Reading

References

Oct 18, 1977·Biochemistry·L Josephson, L C Cantley
May 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·I R GibbonsW J Tang
Jan 11, 1979·Nature·U SchwabeE Söchtig
Jan 23, 1979·Biochemistry·G H Bond, P M Hudgins
May 28, 1979·Biochemical and Biophysical Research Communications·G GruppA Schwartz
Feb 21, 1973·Journal of the American Chemical Society·N D ChasteenM W Hanna
Feb 27, 1974·Biochemical and Biophysical Research Communications·J L JohnsonK V Rajagopalan
Sep 1, 1973·Analytical Biochemistry·R E BeneschS Yung
Dec 26, 1973·Journal of the American Chemical Society·R N LindquistG E Lienhard
Sep 20, 1972·Journal of the American Chemical Society·E B Paniago, D W Margerum
Apr 6, 2011·Proceedings of the National Academy of Sciences of the United States of America·Ryan T HietpasDaniel N A Bolon

Citations

Jan 1, 1983·Molecular and Cellular Biochemistry·M Ramanadham, M Kern
Dec 6, 1995·Molecular and Cellular Biochemistry·D C CransA D Keramidas
Dec 6, 1995·Molecular and Cellular Biochemistry·M J KingR K Sharma
Aug 1, 1988·Biological Trace Element Research·E MacDonaldH Komulainen
Jan 1, 1989·Biological Trace Element Research·C H Hill
Jul 1, 1989·Biological Trace Element Research·P RichelmiG Bellomo
Jun 1, 1994·Biological Trace Element Research·A M CortizoS B Etcheverry
Aug 1, 1991·Biological Trace Element Research·E G FerrerE J Baran
Aug 3, 2011·Biological Trace Element Research·Yuanxin DengXiaodong Liu
Sep 2, 2011·Biological Trace Element Research·Juan LiuKangping Wang
Jan 1, 1985·The International Journal of Biochemistry·S Colombatto, M A Grillo
Jan 1, 1983·Toxicology·M HeideG F Fuhrmann
Feb 1, 1990·Toxicology Letters·J Owusu-YawC I Wei
Nov 1, 1981·Hearing Research·D C MarcusR Thalmann
Jan 1, 1991·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·H Zaporowska, W Wasilewski
Jun 1, 1992·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·H Zaporowska, W Wasilewski
Aug 4, 1998·General Pharmacology·M KirkovaE Russanov
Mar 1, 1997·Trends in Endocrinology and Metabolism : TEM·E Tsiani, I G Fantus
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J B Smith
Oct 1, 1983·The Journal of Cell Biology·L H EnglishL C Cantley
Aug 15, 1986·European Journal of Biochemistry·A J Driessen, W N Konings
Nov 4, 2004·Biochemical and Biophysical Research Communications·Ravi VijayvargiaM V Krishnasastry
Jan 1, 1993·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·H ZaporowskaM Słotwińska
Oct 20, 2005·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Hesheng OuMatthew J Brady
Oct 21, 2011·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Tong-Tong LiuKui Wang
Jul 21, 1997·FEBS Letters·M Solioz, J Camakaris
Dec 6, 2011·Blood Cells, Molecules & Diseases·Poul BennekouLars Ole Simonsen
Jul 27, 2011·Environmental Toxicology and Pharmacology·Agnieszka Scibior, H Zaporowska
Mar 14, 2007·Journal of Inorganic Biochemistry·S S SoaresM Aureliano
Sep 1, 1985·Archives of Biochemistry and Biophysics·D M Delfert, J M McDonald
Aug 31, 1982·Biochemical and Biophysical Research Communications·H SakuraiS Shimomura
Feb 28, 1990·Biochimica Et Biophysica Acta·H P BodeG F Fuhrmann
Oct 19, 2011·Bioinorganic Chemistry and Applications·R FrancikT Librowski
Sep 30, 1982·Biochemical and Biophysical Research Communications·H SakuraiS Shimomura
May 15, 1991·Archives of Biochemistry and Biophysics·P J StankiewiczA J Davison
Sep 1, 1987·Brain Research·S AmirY Shechter
Apr 1, 1984·Biochemical Pharmacology·E ErdmannH Scholz
Jan 13, 2015·Journal of Inorganic Biochemistry·Kenneth Kustin
Apr 22, 2015·Environment International·Muhammad ImtiazShuxin Tu
Jan 24, 1998·Archives of Biochemistry and Biophysics·T KrishnamoorthyK V Ramaiah
Aug 26, 2004·Biochimica Et Biophysica Acta·H EbelT Günther
Dec 22, 1999·Cell Calcium·P J Romero, E A Romero
Dec 1, 1991·Archives of Biochemistry and Biophysics·G L Mendz
Jan 11, 1984·Biochimica Et Biophysica Acta·G F FuhrmannP A Knauf
Nov 26, 1986·Biochemical and Biophysical Research Communications·M S PatoleT Ramasarma
Aug 10, 1983·Biochimica Et Biophysica Acta·M HatchD L Earnest
Sep 18, 2013·Inorganic Chemistry·D C CransEugenijus Norkus
Dec 19, 2001·The Journal of Experimental Zoology·E E Faszewski, J G Kunkel
Jul 14, 1998·American Journal of Hematology·H T TerraS T Saad
Dec 13, 2016·Drug and Chemical Toxicology·Rodrigo Anibal Mateos-NavaMario Agustín Altamirano-Lozano
May 1, 2010·Biomolecular Concepts·Hitoshi Michibata, Tatsuya Ueki
Jul 14, 1987·Biochemistry·J NievesM Martinez-Maldonado
Oct 17, 1981·Lancet·A Whyte
Jun 11, 1992·Biochimica Et Biophysica Acta·H PlassK Turnheim
Jan 1, 1990·Archives of Biochemistry and Biophysics·G L MendzP W Kuchel
Dec 2, 2004·Canadian Journal of Physiology and Pharmacology·Rajendra K Sharma
Jun 24, 2017·Dalton Transactions : an International Journal of Inorganic Chemistry·Daniele SannaEugenio Garribba
May 1, 1981·Journal of Neurochemistry·V Adám-ViziP Simon
Sep 23, 2009·Dalton Transactions : an International Journal of Inorganic Chemistry·Susana RamosM Aureliano
Sep 11, 2008·Expert Opinion on Therapeutic Targets·Md Shenuarin BhuiyanKohji Fukunaga
Nov 15, 2000·Canadian Journal of Physiology and Pharmacology·M C CamJ H McNeill
Mar 29, 2014·Dalton Transactions : an International Journal of Inorganic Chemistry·Yutaka YoshikawaEugenio Garribba
Sep 1, 1983·British Journal of Pharmacology·R A Nayler, M P Sparrow
Sep 15, 2020·Chemical Communications : Chem Comm·Giuseppe Sciortino, Eugenio Garribba
Feb 1, 1997·FEMS Microbiology Letters·I MannazzuF Fatichenti
May 11, 2013·Dalton Transactions : an International Journal of Inorganic Chemistry·Hiroki KitayamaTatsuya Ueki
Nov 1, 1982·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·L P Rybak, T Morizono
Apr 29, 2016·Metallomics : Integrated Biometal Science·Daniele SannaEugenio Garribba

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Eryhem
Oxidation-Reduction
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