PMID: 11604559Oct 18, 2001Paper

Glutathione-S-transferase-pi expression regulates sensitivity to glutathione-doxorubicin conjugate

Anti-cancer Drugs
K TashiroY Ishibashi

Abstract

We have reported that glutathione-doxorubicin conjugate (GSH-DXR) exhibited potent cytotoxicity against tumor cells and inhibited glutathione-S-transferase (GST) enzyme activity. In order to determine whether or not the expression of GST-pi lowered the cytotoxicity of GSH-DXR, cytocidal activity of the conjugate was examined using tumor cells in which the level of GST-pi expression was regulated by transfecting GST-pi cDNA in the correct or reverse direction and comparing with that of DXR. Enhancement of GST-pi expression by transfecting GST-pi sense cDNA into human hepatoblastoma HepG2 cells in which GST-pi expression was extremely low caused an increase in GST activity from 0.26 to 55.0 nmol/mg/min and a marked reduction in transfectant sensitivity to GSH-DXR to 1/120 (0.15-18 nM IC50) although the sensitivity to DXR was slightly decreased to 1/2.6 (380-990 nM IC50). By contrast, a high GST-pi-expressing human colon cancer cell line, HT29, showed a decrease in GST enzyme activity from 72.0 to 45.9 nmol/mg/min after transfecting GST-pi antisense cDNA and a marked improvement in transfectant sensitivity to GSH-DXR was observed (28-2.9 nM IC50) compared with the transfectant sensitivity to DXR (1020-320 nM IC50). Additionally, t...Continue Reading

References

Jan 1, 1991·Life Sciences·P De IsabellaF Zunino
Jan 1, 1989·Annual Review of Biochemistry·J A Endicott, V Ling
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·D J FitzGeraldI Pastan
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K H CowanC E Myers
Oct 1, 1970·Analytical Biochemistry·P C Jocelyn, A Kamminga
Oct 1, 1993·British Journal of Haematology·M PetriniG Ronca
Mar 4, 1999·The EMBO Journal·V AdlerZ Ronai
Jun 9, 1999·British Journal of Cancer·T AsakuraK Ohkawa

❮ Previous
Next ❯

Citations

Mar 27, 2019·Cancers·Jose J G MarinRocio I R Macias
Mar 24, 2007·Journal of Biomolecular Screening·Jeroen KoolNico P E Vermeulen
Oct 23, 2014·Archives of Pharmacal Research·Jong Min ChoiSang Kyum Kim

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Toxicological Sciences : an Official Journal of the Society of Toxicology
Grace E KisslingGary A Boorman
Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
Y F Wang, M L Hu
Xenobiotica; the Fate of Foreign Compounds in Biological Systems
Q FengT Osawa
Mutation Research
Fekadu KassieS Knasmüller
© 2021 Meta ULC. All rights reserved