Glutathione S-transferase-pi overexpression is closely associated with K-ras mutation during human colon carcinogenesis

Gastroenterology
Koji MiyanishiY Niitsu

Abstract

In colorectal adenoma and carcinoma, glutathione S-transferase-pi (GSTP1-1) is highly expressed. K-ras mutation is also known to occur frequently in colorectal adenoma and carcinoma, as well as in the putative precursor of adenoma, aberrant crypt foci (ACF). Further, forced expression of v-H-ras in rat liver epithelial cells has been shown to enhance rat pi-class GST expression. The aim of the present study is, therefore, to investigate the causative relationship between GSTP1-1 overexpression and K-ras mutation in these lesions. Twenty-seven specimens of colorectal carcinoma, 24 of adenoma, and 28 of ACF were examined in this study. The expression of GSTP1-1 or p21(K-ras) was examined by immunohistochemistry. The GSTP1-1 messenger RNA levels were measured by TaqMan reverse-transcription polymerase chain reaction (PCR). K-ras mutation was detected by two-step PCR restriction fragment length polymorphism. v-K-ras transfection to RPMI-4788 colon carcinoma cells was carried out by the lipofection method. Activities of GSTP1-1 promoters containing AP-1 and Sp1 responsive elements in the v-K-ras transfectants were measured by a secreted form of human placental alkaline phosphatase (SEAP) assay. Nuclear protein from these transfectan...Continue Reading

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Citations

Dec 4, 2003·The British Journal of Nutrition·Gabriele Beyer-SehlmeyerBeatrice L Pool-Zobel
Jul 29, 2020·Proceedings of the National Academy of Sciences of the United States of America·Yoshiro NiitsuIrving Listowsky
Sep 15, 2004·British Journal of Cancer·A LosadaL F García-Fernández
Dec 15, 2018·Chembiochem : a European Journal of Chemical Biology·Yuko ShishidoHiroshi Abe
Mar 7, 2012·Colorectal Disease : the Official Journal of the Association of Coloproctology of Great Britain and Ireland·M R BedfordD Burke
Jan 21, 2012·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·Junwei ZongJianwu Tang
Jun 17, 2015·American Journal of Physiology. Lung Cellular and Molecular Physiology·Marianne E FletcherNandor Marczin
Dec 25, 2019·The Journal of Clinical Investigation·Kewen HuXiufeng Pang
Apr 23, 2020·International Journal of Oncology·Jian CuiLan Yi
Nov 18, 2011·Journal of Human Genetics·Yasumitsu MoriyaNaohiko Seki
Mar 11, 2015·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Toshifumi HokiJunji Kato
Oct 13, 2007·Nihon Naika Gakkai zasshi. The Journal of the Japanese Society of Internal Medicine·Yoshiro Niitsu, Tetsuji Takayama
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Nov 20, 2019·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Jolantha BeyerleCornelia M Ulrich
Nov 22, 2002·Journal of Dermatological Science·Yuesheng ZhangMin Jian Xu
Jun 5, 2004·Toxicology Letters·Beate PeschYon-Dschun Ko

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