Glutathione S-transferases of Aulacorthum solani and Acyrthosiphon pisum: partial purification and characterization

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
Frédéric FrancisPaul Dierickx

Abstract

Glutathione S-transferases (GST) play an important role in the detoxification of many substances including allelochemicals from plants. Brassicaceae plants contain glucosinolates and emit volatile isothiocyanates which affect the GST system. A comparison of the GST of two aphid species, the generalist Aulacorthum solani found on Brassicaceae and the Fabaceae specialist Acyrthosiphon pisum, was made to try to explain their respective feeding behaviour. Differences of GST were determined among the two aphid species based on purification by affinity chromatography, SDS-PAGE and on kinetic studies. Purification yields using an epoxy-activated Sepharose 6B column were highly different for the two aphid species (18% and 34% for A. solani and A. pisum, respectively). These variations were confirmed by SDS-PAGE. While only a 27-kDa band was observed for A. pisum, two bands of approximately 25-kDa were visualized for the generalist aphid, A. solani. Considering the kinetic results, differences of Km and Vmax were observed following the aphid species when a range of substrates (CDNB and DCNB) and GSH concentrations were tested. Studies on the detoxification enzymes of generalist and specialist herbivores would be undertaken to determine ...Continue Reading

References

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Citations

Aug 15, 2006·Journal of Chemical Ecology·Yasmin J CardozaOwain R Edwards
May 29, 2003·Journal of Insect Physiology·S MukanganyamaH M Niemeyer
Dec 4, 2015·Insect Biochemistry and Molecular Biology·Xiaopeng ZouSichun Zheng
Aug 14, 2012·Archives of Insect Biochemistry and Physiology·Henry Ofosuhene SintimNaoki Motoyama
Feb 18, 2005·Archives of Insect Biochemistry and Physiology·Frédéric FrancisEric Haubruge
Feb 28, 2015·Journal of Proteome Research·Ritu ChaudharyIsgouhi Kaloshian
Feb 15, 2011·Phytochemistry·Inis Winde, Ute Wittstock

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