Glutathione supplementation to semen extender improves the quality of frozen-thawed canine spermatozoa for transcervical insemination

The Journal of Reproduction and Development
Kazuko OgataYoshikazu Nagao

Abstract

The present study was conducted to evaluate whether supplementation of semen extender with glutathione (GSH) can maintain the quality of frozen-thawed canine spermatozoa. Eighteen ejaculates were obtained from 5 dogs and placed in extender (20% egg yolk, Tris, citric acid, lactose, raffinose, antibiotics and 6.5% glycerol) containing 0 (control), 2.5, 5, 7.5 or 10 mM GSH. The samples were cooled to 4 C and then frozen in liquid nitrogen vapor. Motility parameters of the sperm were evaluated at 0, 1, 2, 3, 4, 12 and 24 h after thawing. Sperm motility was higher in the 5 mM GSH group than in the control or 2.5 and 10 mM GSH groups; this effect was observed at 1 to 24 h after thawing (P < 0.05). The 5 mM GSH group had a higher sperm viability index at 12 and 24 h after thawing compared with the other groups (P < 0.05). Acrosome integrity, evaluated at 4 h after thawing, was greater in two of the GSH-treated groups (5 and 10 mM) compared with the control. Lipid peroxidation (LP) levels immediately after thawing were lower in the 5 and 10 mM GSH groups compared with the control, while those at 12 h after thawing did not differ significantly. Frozen-thawed semen in the 5 mM GSH group was used for transcervical insemination of 4 bitch...Continue Reading

References

Jan 1, 1995·Annual Review of Medicine·J H Southard, F O Belzer
Jan 27, 1998·Genomics·C S MellershE A Ostrander
Mar 31, 2000·Human Reproduction·L GandiniA Lenzi
Aug 5, 2000·Animal Reproduction Science·S Salamon, W M Maxwell
Jan 5, 2002·The Journal of Veterinary Medical Science·Y IchikawaH Takahashi
Dec 31, 2002·The Veterinary Journal·A R SilvaL D MacHado da Silva
Oct 17, 2003·Biology of Reproduction·J RivlinH Breitbart
Dec 4, 2003·Free Radical Biology & Medicine·Jos F H M Brouwers, Barend M Gadella
Mar 25, 2004·Reproductive Biology and Endocrinology : RB&E·Dorota Sanocka, Maciej Kurpisz
Dec 14, 2004·Theriogenology·Gaia C LuvoniDebora Macis
Feb 24, 2005·Theriogenology·Johan Oliviette Nöthling, Rachel Shuttleworth
Aug 9, 2005·Animal Reproduction Science·S A Meyers
Jan 18, 2006·Molecular and Cellular Endocrinology·R John Aitken, Mark A Baker
Jul 21, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·A M PetrunkinaE Töpfer-Petersen
Jun 3, 2009·Journal of Forensic Sciences·Sree KanthaswamyMikko T Koskinen
Sep 17, 2009·Reproduction in Domestic Animals = Zuchthygiene·J C MonteiroC I Vannucchi
May 7, 2010·Zygote : the Biology of Gametes and Early Embryos·Yoshikazu NagaoYoku Kato
Oct 12, 2010·Biology of Reproduction·Yasuyuki AbeHiroshi Suzuki
Apr 15, 2014·BioMed Research International·Maja Zakošek PipanPetra Zrimšek
Jun 21, 2014·Reproduction in Domestic Animals = Zuchthygiene·T RijsselaereA Van Soom
Jun 11, 2009·Reproductive Medicine and Biology·Yoshinori SuwaHiroshi Suzuki

❮ Previous
Next ❯

Methods Mentioned

BETA
Assay
enzyme-linked fluorescence assay
PCR
MDA

Software Mentioned

StatView

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.