Glutathione transferases with vanadium-binding activity isolated from the vanadium-rich ascidian Ascidia sydneiensis samea

Biochimica Et Biophysica Acta
Masafumi YoshinagaH Michibata

Abstract

Some ascidians accumulate vanadium in vanadocytes, which are vanadium-containing blood cells, at high levels and with high selectivity. However, the mechanism and physiological significance of vanadium accumulation remain unknown. In this study, we isolated novel proteins with a striking homology to glutathione transferases (GSTs), designated AsGST-I and AsGST-II, from the digestive system of the vanadium-accumulating ascidian Ascidia sydneiensis samea, in which the digestive system is thought to be involved in vanadium uptake. Analysis of recombinant AsGST-I confirmed that AsGST-I has GST activity and forms a dimer, as do other GSTs. In addition, AsGST-I was revealed to have vanadium-binding activity, which has never been reported for GSTs isolated from other organisms. AsGST-I bound about 16 vanadium atoms as either V(IV) or V(V) per dimer, and the apparent dissociation constants for V(IV) and V(V) were 1.8 x 10(-4) M and 1.2 x 10(-4) M, respectively. Western blot analysis revealed that AsGSTs were expressed in the digestive system at exceptionally high levels, although they were localized in almost all organs and tissues examined. Considering these results, we postulate that AsGSTs play important roles in vanadium accumulati...Continue Reading

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Citations

Jul 20, 2012·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Satoshi KumeHitoshi Michibata
Sep 18, 2009·Cellular and Molecular Life Sciences : CMLS·Vincent LaizéM Leonor Cancela
May 1, 2010·Biomolecular Concepts·Hitoshi Michibata, Tatsuya Ueki
Jan 10, 2019·Current Medicinal Chemistry·Agnieszka Ścibior, Joanna Kurus
May 8, 2010·Dalton Transactions : an International Journal of Inorganic Chemistry·Manuel Aureliano

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