Glutathionylation of dengue and Zika NS5 proteins affects guanylyltransferase and RNA dependent RNA polymerase activities

PloS One
Chonticha SaisawangAlbert J Ketterman

Abstract

It has been estimated for dengue infection that the global population at risk is 3.5 billion people, which makes dengue an important public health problem. The causative agents of dengue are dengue viruses. For dengue virus replication, the dengue virus NS5 protein is of special importance as it has several enzyme activities important for viral replication. Previous reports of phosphorylation and SUMOylation of dengue NS5 have shown these protein modifications have important consequences for NS5 functions. In this report we identify glutathionylation, another reversible post translation modification that impacts on NS5 enzyme activity. Using dengue virus infected cells we employed specific antibodies and mass spectrometry to identify 3 cysteine residues of NS5 protein as being glutathionylated. Glutathionylation is a post translational protein modification where glutathione is covalently attached to a cysteine residue. We showed glutathionylation occurs on 3 conserved cysteine residues of dengue NS5. Then we generated two flavivirus recombinant full length proteins, dengue NS5 and Zika NS5, to characterize two of the NS5 enzyme activities, namely, guanylyltransferase and RNA-dependent RNA polymerase activities. We show glutathi...Continue Reading

References

Sep 15, 2014·Biochemical and Biophysical Research Communications·Xiyuan LiuMinsun Chang

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Citations

Aug 23, 2019·Nutrients·Paola ChecconiAnna Teresa Palamara
Nov 7, 2019·Oxidative Medicine and Cellular Longevity·Marina K KukhanovaAlexander V Ivanov
Jan 20, 2019·International Journal of Molecular Sciences·Zheng ZhuJasper Fuk-Woo Chan
Dec 19, 2018·Viruses·Stephanie ThurmondRong Hai

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Datasets Mentioned

BETA
AFD30972.1

Methods Mentioned

BETA
GTase
GTases
immunoprecipitation
pull down
PCR
guanylylation

Software Mentioned

Azure

Related Concepts

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