PMID: 8942988Nov 26, 1996Paper

Gly-63-->Gln substitution adjacent to His-64 in rodent carbonic anhydrase IVs largely explains their reduced activity

Proceedings of the National Academy of Sciences of the United States of America
S TamaiWilliam S Sly

Abstract

Carbonic anhydrase (CA) IV is a glycosyl-phosphatidylinositol-anchored isozyme expressed on plasma membranes of capillary endothelial cells and certain epithelial cells of the nephron, the colon, and the genitourinary tract. CA IVs purified from bovine and rabbit lungs are high-activity enzymes, like human CA IV, while CA IV from mouse and rat lungs had only 10-20% as much catalytic activity. To explain the molecular basis for these differences in activity, we isolated and characterized the full-length cDNAs for bovine and rabbit CA IVs and compared their sequences to those we previously reported for human, murine, and rat CA IVs. These comparisons led us to postulate that a Gly-63-->Gln substitution adjacent to His-64 in the rodent enzymes accounts for their lower activity. To test this hypothesis, we made the Gly-63-->Gln mutants of bovine and rabbit CA IVs and the Gln-63-->Gly mutant of murine CA IV by site-directed mutagenesis, and compared the activities of mutant and wild-type CA IVs expressed in COS-7 cells. In addition, we produced recombinant cDNAs expressing secretory forms of the Gly-63 and Gln-63 forms of each of the three enzymes and compared the activities of the enzymes purified from transfected COS-7 cell secret...Continue Reading

References

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Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·T StamsD W Christianson

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Citations

Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·O TüreciW S Sly
Dec 14, 2006·Kidney International·J M Purkerson, G J Schwartz
Oct 5, 2017·European Biophysics Journal : EBJ·Aurelija MickevičiūtėDaumantas Matulis
Jun 3, 2000·American Journal of Physiology. Renal Physiology·G J SchwartzS Vijayakumar
Feb 24, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·O RosenL P Brion
May 23, 1997·The Journal of Biological Chemistry·J D HurtD N Silverman
Apr 13, 1999·The American Journal of Physiology·G J SchwartzW S Sly
Dec 8, 2004·The Journal of Experimental Biology·Terri J SeronPaul J Linser

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