Glycan-foraging systems reveal the adaptation of Capnocytophaga canimorsus to the dog mouth

MBio
Francesco RenziGuy R Cornelis

Abstract

Capnocytophaga canimorsus is known to form two kinds of cells on blood agar plates (coccoid and bacillary), evoking phase variation. When grown in coculture with animal cells these bacteria appeared only as bacilli, but in the presence of vancomycin they were round, indicating that coccoid shapes likely result from weakening of the peptidoglycan layer. Polysaccharide utilization locus 5 (PUL5) and sialidase mutant bacteria, unable to retrieve glycans from glycoproteins, grew less than wild-type bacteria and also appeared polymorphic unless GlcNAc was added, suggesting that C. canimorsus is unable to synthesize GlcNAc, an essential component of peptidoglycan. Accordingly, a genome analysis was conducted and revealed that C. canimorsus strain 5 lacks the GlmM and GlmU enzymes, which convert glucosamine into GlcNAc. Expression of the Escherichia coli GlmM together with the acetyltransferase domain of GlmU allowed PUL5 mutant bacteria to grow normally, indicating that C. canimorsus is a natural auxotroph that relies on GlcNAc harvested from the host N-glycoproteins for peptidoglycan synthesis. Mucin, a heavily O-glycosylated protein abundant in saliva, also rescued growth and the shape of PUL5 mutant bacteria. Utilization of mucin ...Continue Reading

References

Jan 1, 1977·Annals of Internal Medicine·T ButlerR B Kohler
Apr 1, 1976·American Journal of Clinical Pathology·R A Bobo, E J Newton
Nov 20, 1998·The Journal of Infection·P BlancheD Sicard
Jul 31, 1998·Protein Science : a Publication of the Protein Society·S BaumgartnerP Bucher
Mar 29, 2003·Science·Jian XuJeffrey I Gordon
Jul 20, 2004·Proceedings of the National Academy of Sciences of the United States of America·Koichiro TamuraSudhir Kumar
Feb 4, 2006·Journal of Clinical Microbiology·David M TierneyJason L Sanchez
Feb 24, 2006·Emerging Infectious Diseases·J Michael JandaWill S Probert
Jan 6, 2007·The Journal of Infectious Diseases·Hwain ShinGuy R Cornelis
Feb 13, 2008·FEMS Microbiology Reviews·Hélène BarreteauDidier Blanot
Aug 30, 2008·Applied and Environmental Microbiology·Manuela Mally, Guy R Cornelis
Nov 14, 2008·Nucleic Acids Research·J R ColeJ M Tiedje
Dec 4, 2008·Proceedings of the National Academy of Sciences of the United States of America·Kerwyn Casey HuangNed S Wingreen
Mar 10, 2009·Veterinary Microbiology·Wim Gaastra, Len J A Lipman
Jun 26, 2009·The Journal of Biological Chemistry·Eric C MartensJeffrey I Gordon
Apr 27, 2010·FEBS Letters·Luciane V MelloDaniel J Rigden
Jan 18, 2011·Veterinary Microbiology·S K DileggeE R Leadbetter
Sep 15, 2011·Journal of Bacteriology·Pablo ManfrediGuy R Cornelis
Apr 19, 2012·Biology Direct·Grzegorz M BoratynThomas L Madden
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Dec 18, 2013·Current Biology : CB·Seth Rakoff-NahoumLaurie E Comstock
Jul 6, 2014·The Journal of Biological Chemistry·Ulrich ZähringerGuy R Cornelis
Nov 5, 2014·Infection and Immunity·Pablo ManfrediGuy R Cornelis

❮ Previous
Next ❯

Citations

Nov 1, 2017·Nucleic Acids Research·Nicolas TerraponBernard Henrissat
Oct 1, 2015·Emerging Microbes & Infections·Francesco RenziGuy Richard Cornelis
Feb 1, 2017·Journal of Bacteriology·Julie M GrondinHarry Brumer
Jun 7, 2015·Applied and Environmental Microbiology·Taichi InuiA Bryan Hanley
Nov 1, 2016·MBio·Frédéric LauberFrancesco Renzi
Jan 24, 2018·Veterinary Ophthalmology·Eric C LedbetterMichele L Edelmann
May 6, 2019·Nature Communications·Pascal LapébieBernard Henrissat
May 2, 2019·Geriatric Orthopaedic Surgery & Rehabilitation·Blane C KellyWilliam Foster
Jan 23, 2021·Anaerobe·Tomaž Accetto, Gorazd Avguštin

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
glycosylation
tandem affinity purification
scraping
PCRs
PCR
pulldowns
affinity purification
affinity column purifications
pulldown

Software Mentioned

Zeiss
BLAST
Zen
Delta BLAST

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.