Glycated fetal calf serum affects the viability of an insulin-secreting cell line in vitro

Metabolism: Clinical and Experimental
Giorgio Luciano VivianiP Odetti

Abstract

The purpose of the present study was to evaluate the direct effects of advanced glycation end products (AGEs) on beta-cells by their exposure to a glycated serum to estimate the cellular viability and the related insulin secretion. Glycation of fetal calf serum was obtained by incubation with 50 mol/L ribose at 37 degrees C for 7 days; at the end of this incubation period, the pentosidine content ranged between 15 and 16 x 10(5) pmol/L. HIT-T15 cells, a pancreatic islet cell line, were grown and cultured for 5 days in Roswell Park Memorial Institute (RPMI) medium containing either not glycated (NGS) or glycated (GS) fetal calf serum. Cellular oxidative stress (ie, thiobarbituric acid-reactive substances) was assessed by high-performance liquid chromatography. Cellular viability was evaluated by detection of proliferation, cell necrosis, and cell apoptosis rate. The insulin secretion and the related intracellular content were evaluated by enzyme-linked immunosorbent assay. The present study reported, after 5 days of exposure to the glycation environment, a moderately reduced cellular proliferation (-20.44% +/- 2.92%) with a corresponding increase of cell necrosis (+67.7% +/- 1.56%) and cell apoptosis (+39.83% +/- 2.92%) rate in ...Continue Reading

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Citations

Nov 13, 2013·European Biophysics Journal : EBJ·Mohd Shahnawaz KhanJaved Musarrat
Jun 6, 2013·Mediators of Inflammation·Alessandra PudduGiorgio L Viviani
Dec 7, 2013·Mediators of Inflammation·Alessandra PudduGiorgio L Viviani
Mar 19, 2015·Biomolecules·Kerstin NowotnyTilman Grune
Nov 20, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Izabela Sadowska-BartoszGrzegorz Bartosz
Jan 16, 2016·Experimental Eye Research·Alessandra PudduMassimo Nicolò
Jan 26, 2012·Biochimica Et Biophysica Acta·Yan WeiRong Qiao He
Apr 1, 2010·Biochemical and Biophysical Research Communications·A PudduG L Viviani
Nov 30, 2010·Biochemical and Biophysical Research Communications·Yunxia ZhuXiao Han
Jul 30, 2015·Journal of Diabetes Research·Alessandra PudduGiorgio Luciano Viviani
Sep 11, 2016·Current Diabetes Reports·Sherman S LeungDanielle J Borg
Mar 7, 2017·Laboratory Investigation; a Journal of Technical Methods and Pathology·Min LiangFan Fan Hou
Dec 15, 2010·Biogerontology·Patrizio Odetti, Ettore Bergamini
Nov 8, 2019·Biochemical Society Transactions·Antonella Del-CorsoPiero Luigi Ipata

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