Glycation Increases the Risk of Microbial Traversal through an Endothelial Model of the Human Blood-Brain Barrier after Use of Anesthetics

Journal of Clinical Medicine
Veronika WeberKaya Bork

Abstract

The function of the human blood-brain barrier (BBB), consisting mainly of the basement membrane and microvascular endothelial cells, is to protect the brain and regulate its metabolism. Dysfunction of the BBB can lead to increased permeability, which can be linked with several pathologies, including meningitis, sepsis, and postoperative delirium. Advanced glycation end products (AGE) are non-enzymatic, posttranslational modifications of proteins, which can affect their function. Increased AGE levels are strongly associated with ageing and degenerative diseases including diabetes. Several studies demonstrated that the formation of AGE interfere with the function of the BBB and may change its permeability for soluble compounds. However, it is still unclear whether AGE can facilitate microbial traversal through the BBB and how small compounds including anesthetics modulate this process. Therefore, we developed a cellular model, which allows for the convenient testing of different factors and compounds with a direct correlation to bacterial traversal through the BBB. Our results demonstrate that both glycation and anesthetics interfere with the function of the BBB and promote microbial traversal. Importantly, we also show that the ...Continue Reading

References

Jan 1, 1990·Journal of Neural Transmission. General Section·A BhardwajJ Weinberger
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·B FreiB N Ames
Mar 1, 1969·The Journal of Cell Biology·M W Brightman, T S Reese
Jan 1, 1993·Eye·W G John, E J Lamb
Mar 29, 2001·Diabetologia·R SinghL Beilin
Feb 14, 2004·Medical Hypotheses·Cheryl A Krone, John T A Ely
Jun 7, 2008·Drug Metabolism and Drug Interactions·Miklós Péter Kalapos
Jul 8, 2008·Nature Reviews. Microbiology·Kwang Sik Kim
May 12, 2009·BMC Infectious Diseases·Ewout S SchutDiederik van de Beek
Aug 17, 2010·Current Opinion in Cell Biology·Elizabeth S Harris, W James Nelson
Nov 13, 2012·Fluids and Barriers of the CNS·Anny-Claude LuissintPierre-Olivier Couraud
Mar 8, 2013·Dermato-endocrinology·Paraskevi Gkogkolou, Markus Böhm
Jul 28, 2013·Interactive Cardiovascular and Thoracic Surgery·Nina SmulterKarl Gunnar Engström
Jan 30, 2014·Biochemical and Biophysical Research Communications·M Elizabeth MeredithJames M May
Aug 12, 2014·CNS & Neurological Disorders Drug Targets·Hari S SharmaAruna Sharma
Nov 12, 2014·PloS One·Dorit BennmannVinayaga S Gnanapragassam
May 27, 2015·Deutsches Ärzteblatt International·Torsten KratzAlbert Diefenbacher
Jan 9, 2016·Acta Neuropathologica·Kelly S DoranPeter Valentin-Weigand
Jan 23, 2016·Nature Reviews. Drug Discovery·William A Banks
Feb 24, 2016·Indian Journal of Endocrinology and Metabolism·Sanjay KalraHarshad Malve
Mar 16, 2016·Acta Neuropathologica·Alan W J MorrisRoxana O Carare
Mar 16, 2016·Mechanisms of Ageing and Development·Maryam HussainRüdiger Horstkorte
Nov 16, 2016·Scientific Reports·Kiril E B van VeenDiederik van de Beek
Jun 9, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jérôme RoyJetty Chung-Yung Lee
Jan 27, 2018·Cell Death & Disease·Caroline Maria Oliveira VolpeJosé Augusto Nogueira-Machado
Aug 28, 2018·Experimental Gerontology·Jonas SchefflerRüdiger Horstkorte
Feb 24, 2019·Analytical Biochemistry·Karthik DhananjayanGerald Münch
Sep 6, 2019·Minerva anestesiologica·Victoria WindmannUNKNOWN BIOCOG Consortium
Mar 3, 2020·Journal of Visualized Experiments : JoVE·Veronika WeberHeidi Olzscha

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Datasets Mentioned

BETA
GM2163

Methods Mentioned

BETA
scraping

Software Mentioned

OriginPro2017
ImageLab
Graph Pad Prism

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