PMID: 8611656Apr 12, 1996Paper

Glycation-induced inactivation of malate dehydrogenase protection by aspirin and a lens molecular chaperone, alpha-crystallin

Biochimica Et Biophysica Acta
M M HeathJ J Harding

Abstract

Non-enzymic glycosylation (glycation) of structural proteins has been widely studied as a possible mechanism in the long-term complications of diabetes. Here we show that glycation inactivates malate dehydrogenase. Aspirin affords some protection against the glycation, but alpha-crystallin, a lens protein which appears to act as a molecular chaperone in other systems, is much more effective. For example, 5 mM glucose completely inactivates malate dehydrogenase in four days, and 5 micrograms alpha-crystallin/ml provides complete protection against this inactivation. Fructose, a superior glycating agent, inactivates the enzyme in 24 hours but even so the same low concentration of alpha-crystallin is able to protect 80% of the activity. Other proteins provide no protection at the same concentration. The inactivation of malate dehydrogenase and other enzymes by glycation could play a role in diabetic complications, and molecular chaperones like alpha-crystallin could serve to protect them.

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Citations

Nov 13, 2013·European Biophysics Journal : EBJ·Mohd Shahnawaz KhanJaved Musarrat
Nov 6, 2009·Applied Microbiology and Biotechnology·Jing ZhengZhanglin Lin
Mar 30, 2010·Applied Microbiology and Biotechnology·Zhanglin Lin, Jing Zheng
Jan 29, 2013·Applied Microbiology and Biotechnology·Yu QianZhanglin Lin
Apr 26, 2011·Molecular Biotechnology·Ashok D ChougaleMahesh J Kulkarni
Mar 29, 2003·Brain Research. Brain Research Reviews·Seiji KikuchiKunio Tashiro
May 29, 2002·Free Radical Biology & Medicine·Galen M PieperPiero Del Soldato
Feb 5, 1999·Progress in Retinal and Eye Research·T J Jacob
Apr 27, 1999·Progress in Retinal and Eye Research·B K Derham, J J Harding
Dec 6, 2001·The International Journal of Biochemistry & Cell Biology·Mohammad D BazziAli S Duhaiman
Jun 18, 2002·Ageing Research Reviews·John J Harding
Nov 18, 2009·Biochemistry. Biokhimii︠a︡·D TalukdarS Ray
Oct 7, 2004·Applied and Environmental Microbiology·Shin-Ichi AkazawaYoshiki Tani
Jun 22, 2002·Experimental Eye Research·Lisa M TaylorRoger J W Truscott
Dec 21, 2004·Biogerontology·George Suji, S Sivakami
Jul 1, 1997·Journal of the American Geriatrics Society·A Gafni
Jul 3, 1998·International Journal of Biological Macromolecules·D W Hook, J J Harding
Jun 11, 2002·Biochemical and Biophysical Research Communications·Mohammad D Bazzi
Oct 22, 2003·Archives of Biochemistry and Biophysics·Vincent M Monnier
Feb 6, 2020·Current Diabetes Reviews·Samreen Amani, Shamila Fatima
Jun 3, 2006·Biochimica Et Biophysica Acta·Rukhsana JabeenMohammed Saleemuddin
Sep 12, 2006·Biochimica Et Biophysica Acta·John J Harding, Elena Ganea

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