PMID: 9451470Feb 6, 1998Paper

Glycation of insulin results in reduced biological activity in mice

Acta Diabetologica
Y H Abdel-WahabP R Flatt

Abstract

Bovine insulin was glycated by in vitro incubation with 20-220 mM D-glucose for 1-48 h. The percentage of glycation was dependent on time, glucose concentration, temperature and pH, attaining values up to 28%. Glucose-lowering activities of glycated and control (non-glycated) insulin preparations were assessed in mice by intraperitoneal injection in a 39% (w/v) glucose solution (2 g/kg body weight) at doses of 0.05 and 0.25 units/kg body weight. Injection of glucose alone significantly (P < 0.001) increased plasma glucose concentrations at 30 min. Simultaneous administration of non-glycated insulin with glucose significantly decreased the 30-min glycaemic excursion (P < 0.001) in a dose-dependent manner. Glycated insulin exhibited a significant reduction (P < 0.001) in glucose-lowering activity under these conditions. The relationship between the extent of insulin glycation and glucose-lowering activity at 0.25 units/kg was assessed using five different insulin preparations glycated between 6%-28%. The insulin-induced decrease in plasma glucose at 30 min was inversely related to the extent of glycation (r = 0.99). Glycated insulin (10(-8) and 10(-6) M) also exhibited a significantly reduced (P < 0.05) ability to stimulate gluco...Continue Reading

Citations

May 25, 2010·Analytical and Bioanalytical Chemistry·Sofia GuedesPedro Domingues
Apr 16, 2013·Applied Biochemistry and Biotechnology·Parnian AlaviAli Akbar Moosavi-Movahedi
Sep 18, 2007·Cell Biochemistry and Biophysics·Sudesh VasdevPawan Singal
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