Glycation of macrophages induces expression of pro-inflammatory cytokines and reduces phagocytic efficiency

Aging
Veronika BezoldKaya Bork

Abstract

Glycation and the accumulation of advanced glycation end products (AGEs) are known to occur during normal aging but also in the progression of several diseases, such as diabetes. Diabetes type II and aging both lead to impaired wound healing. It has been demonstrated that macrophages play an important role in impaired wound healing, however, the underlying causes remain unknown. Elevated blood glucose levels as well as elevated methylglyoxal (MGO) levels in diabetic patients result in glycation and increase of AGEs. We used MGO to investigate the influence of glycation and AGEs on macrophages. We could show that glycation, but not treatment with AGE-modified serum proteins, increased expression of pro-inflammatory cytokines interleukin 1β (IL-1β) and IL-8 but also affected IL-10 and TNF-α expression, resulting in increased inflammation. At the same time, glycation reduced phagocytic efficiency and led to impaired clearance rates of invading microbes and cellular debris. Our data suggest that glycation contributes to changes of macrophage activity and cytokine expression and therefore could support the understanding of disturbed wound healing during aging and diabetes.

References

Jan 1, 1992·International Archives of Allergy and Immunology·H Spits, R de Waal Malefyt
Dec 1, 1989·The Journal of Experimental Medicine·D F FiorentinoT R Mosmann
Jan 1, 1994·Annual Review of Medicine·K J Tracey, A Cerami
Jan 1, 1993·Journal of Clinical Laboratory Analysis·J T Wu
Feb 15, 1993·European Journal of Biochemistry·S A Phillips, P J Thornalley
Jan 26, 1999·Annals of the New York Academy of Sciences·C A Dinarello
Feb 28, 2002·Annual Review of Immunology·Charles A Janeway, Ruslan Medzhitov
Jan 14, 2003·Annual Review of Immunology·Kiyoshi TakedaShizuo Akira
Jan 13, 2005·JAMA : the Journal of the American Medical Association·Nalini SinghBenjamin A Lipsky
Apr 16, 2005·Neuro-oncology·Daniel J BratErwin G Van Meir
Jul 23, 2005·Annals of the New York Academy of Sciences·Paul J Thornalley
Oct 18, 2005·Immunity·Alberto MantovaniMassimo Locati
Nov 18, 2005·Lancet·Vincent Falanga
Mar 8, 2006·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Antonio SicaPaola Allavena
Nov 4, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Fernando O MartinezAlberto Mantovani
Oct 1, 1994·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·G D MulderG W Duncan
May 4, 2007·The Journal of Clinical Investigation·Harold Brem, Marjana Tomic-Canic
Jan 26, 2008·Nature Reviews. Immunology·Lynda M Stuart, R Alan Ezekowitz
Sep 5, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marie BenoitJean-Louis Mege
Dec 25, 2008·Annual Review of Immunology·Fernando O MartinezSiamon Gordon
Jul 25, 2009·Transplant International : Official Journal of the European Society for Organ Transplantation·Daniela WeiskopfBeatrix Grubeck-Loebenstein
Nov 15, 2011·Diabetes Research and Clinical Practice·David R WhitingJonathan Shaw
Mar 5, 2013·The Journal of Clinical Investigation·Encarnacion Montecino-RodriguezKenneth Dorshkind
Apr 2, 2013·Journal of Leukocyte Biology·Katrin Kierdorf, Günter Fritz
Apr 16, 2014·BioMed Research International·Cristina AngeloniSilvana Hrelia
Jul 30, 2014·Advances in Therapy·Dimitrios BaltzisAristidis Veves
Aug 28, 2014·Current Opinion in Nephrology and Hypertension·Sandeep K Mallipattu, Jaime Uribarri
Feb 11, 2015·Biochemical and Biophysical Research Communications·Naila Rabbani, Paul J Thornalley
Apr 15, 2015·American Journal of Respiratory Cell and Molecular Biology·Abdullah A TariqueEmmanuelle Fantino
May 12, 2016·Biochimica Et Biophysica Acta·Raquel López-DíezAnn Marie Schmidt
Feb 18, 2017·International Journal of Molecular Sciences·Suzan WetzelsJerome J A Hendriks

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Citations

Apr 8, 2020·Cells·Maximilian ScheerVinayaga Srinivasan Gnanapragassam
Nov 17, 2019·Cells·Zhennan YeOtmar Huber
Aug 17, 2020·Cellular Immunology·Hiba Yaseen, Mogher Khamaisi
Jul 13, 2021·Therapeutic Advances in Infectious Disease·Samuel WindhamKristine M Erlandson
Jul 6, 2021·Frontiers in Immunology·Meirong LiXiaobing Fu

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Methods Mentioned

BETA
flow cytometry
AGE-FCS
FCS
dot blot
PMA
PCR

Software Mentioned

Image J
OriginPro
FCAP

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