Glycation of nail proteins: from basic biochemical findings to a representative marker for diabetic glycation-associated target organ damage

PloS One
Antoine Sadiki KishabongoJoris R Delanghe

Abstract

Although assessment of glycated nail proteins may be a useful marker for monitoring of diabetes, their nature and formation are still poorly understood. Besides a detailed anatomical analysis of keratin glycation, the usefulness of glycated nail protein assessment for monitoring diabetic complications was investigated. 216 patients (94 males, 122 females; mean age ± standard deviation: 75.0 ± 8.7 years) were enrolled. Glycation of nail and eye lens proteins was assessed using a photometric nitroblue tetrazolium-based assay. Following chromatographic separation of extracted nail proteins, binding and nonbinding fractions were analyzed using one-dimensional gel electrophoresis. Using a hand piece containing a latch-type-bur, a meticulous cutting of the nail plate into superficial and deep layers was performed, followed by a differential analysis of fructosamine. Using SDS PAGE, four and two bands were identified among the nonglycated and glycated nail fraction respectively. Significantly lower fructosamine concentrations were found in the superficial nail layer (mean: 2.16 ± 1.37 μmol/g nails) in comparison with the deep layer (mean: 4.36 ± 2.55 μmol/g nails) (P<0.05). A significant higher amount of glycated eye lens proteins was...Continue Reading

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Citations

Mar 30, 2016·International Journal of Biological Macromolecules·Shaziya AllarakhaAsif Ali
Nov 1, 2015·Journal of Diabetes Science and Technology·David C Klonoff
Jul 22, 2018·Acta Clinica Belgica·Justin C CikomolaJoris R Delanghe
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Feb 7, 2016·Journal of Clinical Pathology·Antoine S KishabongoJoris R Delanghe
May 8, 2018·Clinical and Experimental Nephrology·Sigurd DelangheJoris Delanghe
Jul 20, 2021·Journal of Ayurveda and Integrative Medicine·Sanjeev RastogiArindam Bhattacharya
Jul 23, 2021·Clinical and Experimental Dermatology·P D YesudianD de Berker
Nov 3, 2021·Critical Reviews in Clinical Laboratory Sciences·Sarahi Jaramillo OrtizFrédéric J Tessier

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Methods Mentioned

BETA
electrophoresis

Software Mentioned

MedCalc

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