Glycer-AGEs-RAGE signaling enhances the angiogenic potential of hepatocellular carcinoma by upregulating VEGF expression.

World Journal of Gastroenterology : WJG
Junichi TakinoMasayoshi Takeuchi

Abstract

To investigate the effect of glyceraldehyde-derived advanced glycation end-products (Glycer-AGEs) on hepatocellular carcinoma (HCC) cells. Two HCC cell lines (Hep3B and HepG2 cells) and human umbilical vein endothelial cells (HUVEC) were used. Cell viability was determined using the WST-8 assay. Western blotting, enzyme linked immunosorbent assay, and real-time reverse transcription-polymerase chain reactions were used to detect protein and mRNA. Angiogenesis was evaluated by assessing the proliferation, migration, and tube formation of HUVEC. The receptor for AGEs (RAGE) protein was detected in Hep3B and HepG2 cells. HepG2 cells were not affected by the addition of Glycer-AGEs. Glycer-AGEs markedly increased vascular endothelial growth factor (VEGF) mRNA and protein expression, which is one of the most potent angiogenic factors. Compared with the control unglycated bovine serum albumin (BSA) treatment, VEGF mRNA expression levels induced by the Glycer-AGEs treatment were 1.00 ± 0.10 vs 1.92 ± 0.09 (P < 0.01). Similarly, protein expression levels induced by the Glycer-AGEs treatment were 1.63 ± 0.04 ng/mL vs 2.28 ± 0.17 ng/mL for the 24 h treatment and 3.36 ± 0.10 ng/mL vs 4.79 ± 0.31 ng/mL for the 48 h treatment, respectively ...Continue Reading

References

May 29, 1999·Gastroenterology·C A MatteoniA J McCullough
Jul 27, 2001·Diabetes·G MarchesiniN Melchionda
Feb 5, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Shivakumar ChitturiJacob George
Mar 22, 2002·Journal of Internal Medicine·H Vlassara, M R Palace
Mar 29, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Ronnie Tung-Ping PoonJohn Wong
Apr 19, 2002·The New England Journal of Medicine·Paul Angulo
Apr 2, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Giulio MarchesiniMario Rizzetto
Mar 11, 2004·The Journal of Investigative Dermatology·Riichiro AbeHiroshi Shimizu
Oct 14, 2004·Gastroenterology·Jaime BoschUNKNOWN European Study Group on rFVIIa in UGI Haemorrhage
Jan 22, 2005·Carcinogenesis·Rafael MayoralPaloma Martín-Sanz
Dec 13, 2005·Cancer Treatment Reviews·Tong Wu
May 23, 2006·Current Molecular Medicine·Takashi SatoMasayoshi Takeuchi
Sep 5, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Terence K LeeSheung Tat Fan
Jun 15, 2007·Journal of Gastroenterology and Hepatology·Hideyuki HyogoSusumu Tazuma
Jul 31, 2008·World Journal of Gastroenterology : WJG·Asmaa-Ibrahim GomaaSimon-D Taylor-Robinson
Mar 25, 2010·World Journal of Gastroenterology : WJG·Yoshitaka Takuma, Kazuhiro Nouso
May 1, 2010·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Brad Q StarleyStephen A Harrison
Nov 13, 2010·World Journal of Gastroenterology : WJG·Yuhki SakuraokaKeiichi Kubota
Nov 16, 2010·Liver International : Official Journal of the International Association for the Study of the Liver·Stephanie CoulonHans Van Vlierberghe
Dec 24, 2010·Journal of Gastroenterology and Hepatology·Shenghua JieLi Jiang

❮ Previous
Next ❯

Citations

Feb 12, 2014·Laboratory Investigation; a Journal of Technical Methods and Pathology·Ayako OjimaSho-ichi Yamagishi
Mar 19, 2013·Journal of Proteomics·Ioannis P TrougakosVassilis G Gorgoulis
Sep 24, 2015·International Journal of Molecular Sciences·Xuanbin WangFengjun Cao
Jun 20, 2015·Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis·Luciana MoreiraPaulo Pinho e Costa
Sep 25, 2014·Journal of Clinical Laboratory Analysis·Hiromi KanKazuaki Chayama
Apr 19, 2015·European Journal of Medical Research·Sho-ichi Yamagishi, Takanori Matsui
Dec 5, 2014·Rejuvenation Research·Sho-ichi YamagishiKei Fukami
Jun 21, 2017·Nutrients·Masayoshi TakeuchiMikihiro Tsutsumi
Mar 1, 2019·Nutrients·Akiko Sakasai-SakaiMasayoshi Takeuchi
May 24, 2020·International Journal of Molecular Sciences·Ali H El-FarShaker A Mousa
Dec 30, 2014·World Journal of Hepatology·Masayoshi TakeuchiSho-Ichi Yamagishi
Oct 17, 2019·International Journal of Molecular Sciences·Dinali H FernandoChandana B Herath
Jul 15, 2020·International Journal of Molecular Sciences·Akiko Sakasai-SakaiMasayoshi Takeuchi
Oct 19, 2014·Cancer Research·Xuebo ChenBehnam Badie
Feb 12, 2021·Experimental & Molecular Medicine·Wiramon RungratanawanichByoung-Joon Song
May 27, 2017·Seminars in Cancer Biology·Mohammad Imran KhanHasan Mukhtar
Apr 4, 2021·Biomolecules·Masayoshi TakeuchiTamihide Matsunaga
Feb 27, 2017·Biochimica Et Biophysica Acta. General Subjects·Ruyuan DengQing Su
May 7, 2020·Journal of Hepatology·Hui HanNatalia Nieto
Aug 28, 2021·World Journal of Gastroenterology : WJG·Evelyn Nunes Goulart da Silva PereiraRosane Harter Griep

❮ Previous
Next ❯

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.