Glycerol-3-phosphate transporter of Escherichia coli: structure, function and regulation

Research in Microbiology
M Joanne LemieuxDa Neng Wang

Abstract

Glycerol-3-phosphate (G3P) plays a major role in glycolysis and phospholipid biosynthesis in the cell. Escherichia coli uses a secondary membrane transporter protein, GlpT, to uptake G3P into the cytoplasm. The crystal structure of the protein was recently determined to 3.3 A resolution. The protein consists of an N- and a C-terminal domain, each formed by a compact bundle of six transmembrane alpha-helices. The substrate-translocation pore is found at the domain interface and faces the cytoplasm. At the closed end of the pore is the substrate binding site, which is formed by two arginine residues. In combination with biochemical data, the crystal structure suggests a single binding site, alternating access mechanism for substrate translocation, namely, the substrate bound at the N- and C-terminal domain interface is transported across the membrane via a rocker-switch type of movement of the domains. Furthermore, GlpT may serve as a structural and mechanistic paradigm for other secondary active membrane transporters.

References

Jan 1, 1976·Annual Review of Microbiology·E C Lin
Jun 1, 1972·Journal of Bacteriology·P S Venkateswaran, H C Wu
Sep 1, 1973·Journal of Bacteriology·J AdlerM M Dahl
Feb 14, 1968·Journal of Molecular Biology·N R CozzarelliE C Lin
Apr 1, 1994·Microbiology·R P NilssonB Rutberg
Oct 24, 1997·The Journal of Antimicrobial Chemotherapy·P ArcaC Hardisson
Apr 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·S S PaoM H Saier
Dec 5, 1998·The Journal of Biological Chemistry·M C Fann, P C Maloney
Apr 2, 1999·Antimicrobial Agents and Chemotherapy·T HoriiM Ohta
Aug 5, 2000·Journal of Molecular Biology·I T PaulsenM H Saier
Aug 26, 2000·Journal of Bacteriology·M H Saier
Mar 12, 2003·Bioorganic & Medicinal Chemistry·Ahmed El ZoeibyRoger C Levesque
Aug 26, 2003·Antimicrobial Agents and Chemotherapy·Annika I NilssonDan I Andersson
Nov 25, 2003·Protein Science : a Publication of the Protein Society·M Joanne LemieuxDa-Neng Wang
Dec 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Hiroshi Nikaido
Dec 20, 2003·Nature·Henrietta VenterHendrik W Van Veen
Sep 18, 1998·Angewandte Chemie·John E Walker

❮ Previous
Next ❯

Citations

Jul 25, 2006·Infection and Immunity·Dirk HofreuterJorge E Galán
Apr 15, 2008·Applied and Environmental Microbiology·Christophe BordiJohn D Helmann
Jun 10, 2008·Annual Review of Microbiology·Christopher J LawDa-Neng Wang
Oct 23, 2008·Journal of Bioinformatics and Computational Biology·Igor F TsigelnySanjay K Nigam
Sep 8, 2009·Journal of Bacteriology·Alfredo Castañeda-GarcíaJesús Blázquez
Feb 8, 2011·The Science of the Total Environment·Sondre MelandKnut Erik Tollefsen
Jun 21, 2007·Molecular Microbiology·Roger J P DawsonKaspar P Locher
Jun 5, 2012·The EMBO Journal·Nicolae SolcanSimon Newstead
Jan 1, 2014·The FEBS Journal·Daniela CordaStefania Mariggiò
Sep 18, 2012·Biochimica Et Biophysica Acta·Jiangwei Yao, Charles O Rock
Nov 26, 2008·Biochimica Et Biophysica Acta·Yutetsu KurumaPier Luigi Luisi
Aug 29, 2007·Current Opinion in Structural Biology·Kaspar HollensteinKaspar P Locher
Nov 19, 2011·The Journal of Antimicrobial Chemotherapy·Drosos E KarageorgopoulosMatthew E Falagas
Jan 8, 2017·Cold Spring Harbor Perspectives in Medicine·Lynn L Silver
Feb 16, 2017·BioMed Research International·Yasuo OhkoshiShin-Ichi Yokota
Jun 13, 2018·Antimicrobial Agents and Chemotherapy·Q C Truong-BolducD C Hooper
Oct 13, 2018·Drug Development Research·Marko JukičMatej Sova
Sep 6, 2019·Microbiology and Molecular Biology Reviews : MMBR·Zhong PengBin Wu
Mar 19, 2019·Microbial Biotechnology·Ignacio Poblete-CastroPablo I Nikel
Jan 1, 2013·Antibiotics·Alfredo Castañeda-GarcíaAlexandro Rodríguez-Rojas
Apr 19, 2018·Proceedings of the National Academy of Sciences of the United States of America·Ling XuDevin Coleman-Derr
Sep 4, 2020·The EMBO Journal·Changhan LeeJames Ca Bardwell
Jul 27, 2011·Plant Physiology·Madhuvanthi RamaiahKashchandra G Raghothama
Aug 18, 2018·The Journal of Physical Chemistry. B·Eefei ChenDavid S Kliger

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.