PMID: 7580801Nov 1, 1995Paper

Glycerol effect on spiramycin production and valine catabolism in Streptomyces ambofaciens

Current Microbiology
A LounèsP Germain

Abstract

Spiramycin production by Streptomyces ambofaciens in a chemically defined medium, with valine as nitrogen source, was controlled by the nature and the concentration of the carbon source. The production of this antibiotic was better in dextrins than in glycerol-containing medium. The negative effect of glycerol could be attributed in part to an excess of energy and a high specific growth rate. The intracellular ATP content, at the start of spiramycin production, was twofold higher in glycerol than in dextrin-containing medium. Increasing the initial concentrations of glycerol led to an increase in the specific growth rate and a drop in spiramycin production. Comparison between glycerol and a protein synthesis inhibitor effects and the use of resting cell systems (RCS) proved that glycerol exerted both inhibitory and repressive actions on spiramycin production independently from the growth. At the enzymatic level, glycerol interfered with valine catabolism by repressing partially valine dehydrogenase (VDH) and alpha-ketoisovalerate dehydrogenase (KIVDH), generator of spiramycin aglycone precursors.

References

Aug 1, 1978·Antimicrobial Agents and Chemotherapy·Y Aharonowitz, A L Demain
Jul 1, 1977·Analytical Biochemistry·S H Bok, A L Demain
Jun 1, 1992·Applied Microbiology and Biotechnology·A LebrihiP Germain
Nov 1, 1991·FEMS Microbiology Letters·T RezankaJ Kopecký
Jan 1, 1986·Folia Microbiologica·J Neuzil, Z Hostálek
Sep 1, 1995·Canadian Journal of Microbiology·A LounèsP Germain

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